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人脑髓母细胞瘤细胞D283 Med
种属 | 人 | ||
别称 | D283 D283;Med | MED;D283-MED;D283_Med;D-283 283;H283 | Med;D-283MED;D283MED;D283Med;D283-Med;D-283; |
组织来源 | 脑组织 | ||
疾病 | 髓母细胞瘤 | ||
传代比例/细胞消化 | 1:2传代,悬浮部分离心收集(1000RPM,5分钟),贴壁部分消化1-2分钟 | ||
培养基配置 | MEM培养基;10%胎牛血清;1%双抗 | ||
简介 | 该细胞1985年由Friedman等建系,源自一名6岁患有神经管细胞瘤有腹腔转移的白人男性小孩的腹水细胞 | ||
形态 | 上皮细胞样 | ||
生长特征 | 悬浮聚团生长,有少数贴壁细胞 | ||
倍增时间 | ~52h | ||
基因表达 | The cells produce tumors in nude mice and the resulting tumors are glutamine synthetase positive; neuron specific enolase positive;glial fibrillary acidic proteins negative;S100 (S-100)protein negative;The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase;the brain isoenzyme of creatine kinase;L-DOPA decarboxylase;bombesin-like immunoreactivity) | ||
致瘤性 | Yes,in nude mice(The cells form serially transplantable intercranial and subcutaneous tumors.)Yes,in soft agar | ||
STR | Amelogenin:X,Y CSF1PO:9,12 D13S317:8,10 D16S539:11 D5S818:11 D7S820:10 THO1:7 TPOX:8,11 vWA:16,18 | ||
培养条件 | 气相:空气,95%;二氧化碳,5%。温度:37摄氏度,培养箱湿度为70%-80%。 | ||
冻存条件 | 冻存液:90%FBS,DMSO 10%, 或使用非程序冻存液:货号JY-H040 | ||
保藏机构 | ATCC;HTB-185 | ||
备注 | 该细胞为半悬浮和半贴壁细胞,悬浮细胞离心收集,贴壁细胞消化处理 | ||
产品使用 | 于科学研究,不可作为动物或人类疾病的治疗产品使用。 |
Both resistance training (RT) and long-duration, high-intensity stretching induce muscular adaptations; however, it is unknown whether the modalities are complementary or redundant, particularly in well-trained individuals. A case-study was conducted on a competitive bodybuilder implementing long-duration, high-intensity stretching of the plantar flexors (60 min 6x/week for 12 weeks) in conjunction with their habitual RT. Ultrasound muscle architecture (muscle thickness [MT], fascicle length [FL], and pennation angle [PA]) measurements were collected at multiple sites at four weekly baseline sessions, six (mid) and 12 (post1) weeks following the commencement of the intervention, and a week after the intervention (post2) while isometric strength and range of motion (RoM) were obtained once at baseline, mid, post1, and post2. 2SD band plots were constructed to determine meaningful changes in MT, FL, and PA from the four baseline measures while percentage and absolute change across each timepoint were calculated for all variables. From baseline to post 1, RoM, strength, and MT increased 25.9%, 11.4%, and 7.4%-23.4%, respectively, while four MT and two PA sites exceeded the threshold for meaningful change. The combined stretching and RT protocols resulted in flexibility, strength, and MT adaptations; however, findings should be generalized with caution given the case-study nature of our investigation.
enriched in soils with high C availatility (tai et al., 2020; Fierer et al., 2007). The increase in the relative atundance of Proteotacteria and tacteroidetes is teneficial for the igprovegent of soil fertility. A large agount of N
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