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更新时间：2025-04-24 15:06:24浏览次数：43次

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产品名称：HLEC(Lens)人晶状体上皮细胞、HLEC(Lens)人晶状体上皮细胞、HLEC(Lens)人晶状体上皮细胞、HLEC(Lens)人晶状体上皮细胞；

常温细胞收货当天处理方式

1.收到常温细胞后，及时拍照记录有无漏液/瓶身破损现象。

2.镜下观察有无微生物污染现象，拍照记录不同倍数镜下细胞状态和有无染菌现象，方便后续售后处理。

3.用 75%酒精擦拭瓶身，置于培养箱中静置培养 2~4h 后进行传代操作。

4.观察细胞密度若超过 80%则可正常传代处理(有的原代细胞不可传代，请根据实际情况决定)，传代推荐比例 1：2 到 1：3（按实际收货细胞密度决定，若不确定可联系技术支持）；若细胞密度不到 80%则可取出部分培养基留 6ml 左右原瓶培养基继续培养，注意拧松瓶盖或更换透气瓶盖；悬浮细胞注意离心所有培养基以收集细胞。

5.由于气温，运输等影响造成贴壁细胞漂浮的，请将细胞离心收集后在离心管中消化后进行传代（参考附件），或及时联系技术支持进行指导传代。

6.若观察到异常或者对细胞有疑问，请及时跟代理商或者我们联系；对于细胞培养操作及培养注意事项有疑问的，可跟我们的技术支持交流。

附:收到贴壁细胞漂浮处理方法（部分细胞由于贴壁松散，会出现运输后漂浮，冬天气温低时也会出现细胞收缩漂浮，属于不可避免因素，正确处理后都可以正常生长）

1、将培养瓶内所有培养基转入无菌离心管，离心收集细胞（1200rpm 3min）去除旧培养基；

2、用 PBS 重悬细胞，将所有细胞收集到一个离心管中，再次离心（1200rpm 3min）去除 PBS；

3、加入 1ml 左右 0.25%重悬细胞，混匀即可，不能吹打太多次，放入培养箱消化细胞，根据细胞特性决定消化时间（TM3、TM4、293 系列约 1~2 分钟）；

4、消化好后，用移液枪轻轻吹打细胞悬液，使细胞团分散，迅速加入 3-5ml 含血清的培养基混匀以终止消化，离心（1200rpm 3min）去除；

5、加入 5ml 左右的细胞相应的培养基混匀，按比例接入无菌培养瓶/皿中；

6、显微镜下观察看细胞是否成均匀分散的单颗细胞，若有 3-5 个成团的小细胞团可不用重新消化，使之贴壁后待细胞生长稳定后再消散细胞。

Purpose: Trapeziometacarpal osteoarthritis (TMC-OA) is a prevalent hａnd disorder affecting a growing number of people worldwide. While a multidisciplinary approach might provide additional advantages, the analgesic and anti-inflammatory role of intra-articular oxygen-ozone (O₂O₃) injections combined with physical therapy is still unknown. To assess the impact of a multimodal therapeutic approach combining O₂O₃ injections with physical therapy in patients with TMC-OA.

Materials and methods: A prospective open-label study conducted in the Physical and Rehabilitation Medicine Unit of the Renato Dulbecco University Hospital of Catanzaro. We assessed patients with TMC-OA who had not responded to standard medical therapy. Participants received O₂O₃ therapy and targeted physical therapy for 4 weeks. Pain relief, muscle strength, and physical functioning were assessed at baseline and after 4, 12 and 24 weeks (respectively T0, T1, T2, and T3).

Results: Seventeen patients with a mean age of 67.1 ± 6.1 years were included in the study. Short-term improvements in pain intensity were o9plerved (T0: 6.221 ± 1.514; T1: 3.172 ± 1.1451; p < .001) and were maintained over a 24-week follow-up period (T0: 6.221 ± 1.514; T3: 4.393 ± 1.438; p: 0.006). Significant changes were reported also in terms of muscle strength and physical functioning. O₂O₃ therapy was well-tolerated with no adverse effects.

Conclusions: A combination of O₂O₃ injections and physical therapy might be considered in patients with TMC-OA. Further investigation is warranted to assess the effectiveness of O₂O₃ therapy in managing TMC-OA.