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产品名称:SU-DHL-10 人弥漫大B细胞淋巴瘤细胞、SU-DHL-10 人弥漫大B细胞淋巴瘤细胞、SU-DHL-10 人弥漫大B细胞淋巴瘤细胞、SU-DHL-10 人弥漫大B细胞淋巴瘤细胞;
种属 | 人 |
别称 | Su-DHL-10; SUDHL10; Sudhl10; SUDHL-10; SuDHL 10; Stanford University-Diffuse Histiocytic Lymphoma-10; DHL10 |
组织来源 | 淋巴结 |
疾病 | 大细胞淋巴瘤 |
传代比例/细胞消化 | 1:2传代 |
培养基配置 | RPMI1640 培养基;10%胎牛血清;1%双抗 |
简介 | established from the pleural effusion of a 25-year-old man with B-cell non-Hodgkin lymphoma (B- NHL), described at the time as diffuse histiocytic lymphoma; cell line carries EZH2 Y641F mutation (own results); assigned to GCB-like lymphoma subtype (germinal center B-cell) |
形态 | 淋巴母细胞样 |
生长特征 | 悬浮生长 |
倍增时间 | ~40h |
STR | D5S818: 11, 12 D13S317: 10, 11 D7S820: 8, 11 D16S539: 10, 11 vWA: 17 THO1: 8, 9.3 TPOX: 8 CSF1PO: 10, 13 Amelogenin: XYTPOX: 8 CSF1PO: 10, 13 |
培养条件 | 气相 :空气 ,95% ;二氧化碳 ,5%。 温度 :37摄氏度 ,培养箱湿度为70%-80%。 |
冻存条件 | 冻存液 :90%FBS ,DMSO 10%, 或使用非程序冻存液 :货号JY-H040 |
保藏机构 | ATCC; CRL-2963 |
备注 | 该细胞为悬浮细胞 ,请注意离心收集细胞悬液 ,请勿直接倒掉细胞培养液。 |
产品使用 | 于科学研究 ,不可作为动物或人类疾病的治疗产品使用。 |
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