ICG amine，吲哚菁绿-氨基，ICG-NH2

ICG amine，吲哚菁绿-氨基，ICG-NH2

英文名：ICG amine，ICG-NH2

中文名：吲哚菁绿-氨基

分子式：C47H56N4O4S

分子量：773.04

外观：绿色粉末

溶解度：二氯甲烷

纯度：95%+

结构式：

ICG amine，吲哚菁绿-氨基，ICG-NH2

ICG is a tricarbocyanine-type dye with NIR-absorbing properties (peak absorption around 800 nm) and emission maximum at 807 nm (10% v/vDMSO/PBS). Little absorption in the visible range accounts for the low autofluorescence, tissue absorbance, and scattering at NIR wavelengths (700-900 nm).

The maleimide of the ICG dye offers the opportunity to develop optimal conjugates. Maleimide active group provides an efficient and convenient way to selectively link ICG dye to sulfhydryl groups (free thiol, R-SH) on various substrates (antibodies[1], peptides, proteins, oligonucleotides, small molecule drugs etc.) at neutral (physiological) pH without any activation. Maleimides have very low reactivity with amines, alcohols, and phenols (incl. tyrosine and histidine) and do not react with histidine and methionine thus providing very high labeling selectivity.

It has been reported that conjugation to some antibodies may cause decrease in fluorescence intensity of ICG bound to antibody due to the formation of H-dimers4. However, fluorescence is restored after cell binding and internalization, which can be used to design an activatable NIR fluorescent probe. Fluorescence intensity of the unbound ICG-antibody conjugate can be dramatically increased (up to 50-fold) by addition of SDS and β-mercaptoethanol, which diminishes π- π stacking5.

近红外荧光染料Near IRDyes激发和发射波长和颜色图：

  
ICG衍生物产品：

ICG NHS ester     1622335-40-3

ICG-alkyne        1622335-41-4

ICG Maleimide     2143933-81-5

ICG-azide

ICG-DBCO

ICG Carboxylic acid

ICG  Amine

ICG Thiol 

ICG-CBT