Cyanine5.5 hydrazide,CY5.5酰肼染料

Cyanine5.5 hydrazide,CY5.5酰肼染料

Cy5.5 hydrazide是含羰基的近红外活性染料。酰肼可以与一些羰基化合物迅速反应，例如醛类和酮类，并能形成稳定的腙。该反应可用于标记偶联物的制备。生物分子中的羰基基团可以在氧化应激或蛋白质的脱氨基作用之后自发的形成。或者，醛基可以通过糖蛋白分子糖残基的高碘酸氧化特异生成，例如抗体。抗体可以被高碘酸钠氧化，并且可以被Cy5.5酰肼标记，从而形成Cy5.5酰肼标记的抗体。由于抗体中的糖残基远离可变的表位结合位点，用这种方法标记抗体是很理想的。
               
Cyanine5.5 hydrazide,CY5.5酰肼染料
 
General properties：
Appearance: dark blue powder
Molecular weight: 669.73
Molecular formula: C40H46Cl2N4O
Solubility: moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
 
Spectral properties：
Excitation maximum, nm: 684
Emission maximum, nm:710
Fluorescence quantum yield:0.2
CF260:0.07
CF280:0.03
 
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Sulfo-Cyanine3 azide
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