thermo核酸定量Qubit microRNA Assay Kit

thermo核酸定量Qubit microRNA Assay Kit

货号 单位规格     单价 (CNY)

Q32880 100 assays   1,483.00

Q32881 500 assays   4,537.00

Sample Type (General): FFPE & Fixed Samples, RNA

For Use With (Equipment): Qubit® Fluorometer

Product Line: Qubit™

Product Size: 100 assays

Sample Type (Specific): RNA (<40 bases), Single-Stranded RNA, Single-Stranded RNA (ssRNA), Tissue (FFPE), miRNA

内容及储存

Store in refrigerator (2–8°C) and protect from light.

The Qubit microRNA Assay Kit allows easy and accurate quantification of microRNA (miRNA) using the Qubit Fluorometer, even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. The assay is highly selective for miRNA over rRNA or large mRNA (>1000 bp). Although the reagent is not exclusively selective for miRNA, we have been able to reproducibly quantify miRNA in pure samples at levels as low as 0.5 ng in the assay tube following the supplied protocol below, even in the presence of mRNA.

Features of Qubit microRNA assay:

• Accurately detects as little as 0.5 ng miRNA in the assay tube

• Core dynamic range of 5–500 ng/mL

• Accurate results for sample concentrations from 50 ng/mL to 100 µg/mL

The Qubit microRNA Assay Kit provides concentrated assay reagent, dilution buffer, and pre-diluted miRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer. Because the assay tolerates 1–20 µL of sample in an assay volume of 200 µL, accurate results can be obtained for initial sample concentrations from 50 ng/mL–100 µg/mL.

Which product to choose for microRNA quantitation?

• For 1–20 samples: use this Qubit microRNA Assay Kit with the Qubit Fluorometer

• For 20–2000 samples: use the Quant-iT microRNA Assay Kit with microplate reader

• For >2000 samples: use the Quant-iT microRNA Reagent with microplate reader

Notes:

1. The Qubit microRNA assay file is included on all Qubit 3 and Qubit 4 fluorometers, but may require installation onto the Qubit 2.0 Fluorometer. Uploading new assays to the Qubit 2.0 Fluorometer requires version 3.10 or later of the Qubit firmware.

2. 500 µL thin-walled PCR tubes are required but not included.

用于Qubit荧光计的Qubit试剂盒，配合简单的混合读取模式，均采用相同的操作方式。其中DNA和RNA分析试剂盒仅需孵育2分钟，而蛋白分析试剂盒需要15分钟 。 Qubit 4荧光计自动保存您的数据，您可用USB数据线或U盘导出。 除了常规的 dsDNA，寡核苷酸，总RNA，小RNA和蛋白质的分析试剂盒，在 Ion Personal Genome Machine(PGM) 测序仪上进行测序之前，可使用专门的Ion Sphere Quality Control Kit在Qubit 4荧光计上进行Ion Sphere颗粒质量评估。