Jackson二抗|315-036-046*

Jackson二抗|315-036-046*

通过胃蛋白酶消化全IgG抗体产生F（ab'）2片段抗体，以除去大部分Fc区，同时留下一些铰链区。 F（ab'）2片段具有通过二硫键连接在一起的两个抗原结合Fab部分，因此它们是二价的。 平均分子量约为110kDa。 它们用于特定应用，例如避免二抗与活体细胞结合Fc受体或蛋白A或蛋白G。

基于免疫电泳和/或ELISA，抗体与小鼠IgG重链的Fc部分反应但不与小鼠免疫球蛋白的Fab部分反应。 未检测到针对小鼠IgM或非免疫球蛋白血清蛋白的抗体。 已通过ELISA和/或固相吸附测试抗体以确保与人血清蛋白的小交叉反应，但其可与来自其他物种的免疫球蛋白交叉反应。

Jackson二抗|315-036-046*

Physical State: Freeze-dried solid 
Storage:

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. 
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. 
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) 

Suggested Working Concentration or Dilution Range:

1:500 - 1:5,000 for immunohisto/cytochemistry
1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates
1:10,000 - 1:200,000 for Western blotting with ECL substrates

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.