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CarboPac PA10糖和氨基酸分析色谱柱
用途和特点
AminoPac PA10柱结合阴离子交换，梯度淋洗以及脉冲电化学检测，可以分离多种氨基酸。分离氨基酸的同时，还可检测氨基糖及碳水化合物。此外，还可分离多种糖类，磷酸化氨基酸，以及含硫氨基酸的普通氧化产物，如磺基丙氨酸。其柱填料为疏水性高聚物薄壳型阴离子交换剂，可在pH0-14范围内使用。填料*的pH稳定性允许使用的淋洗液，有助于氨基酸在金电极的阳极氧化。
CarboPac PA10糖和氨基酸分析色谱柱
订货信息：
Dionex CarboPac PA10 糖和氨基酸分析柱
| 分析柱 | |
| CarboPac PA10 Analytical Column (2 x 250 mm) | 057180 |
| CarboPac PA10 Analytical Column (4 x 250 mm) | 046110 |
| CarboPac PA10 Semi-Preparative (9 X 250 mm) | SP4216 |
| 保护柱 | |
| CarboPac PA10 Guard Column (2 x 50 mm) | 057181 |
| AminoTrap Column (2 x 50 mm) | SP5578 |
| CarboPac PA10 Guard Column (4 x 50 mm) | 046115 |
| Amino Trap Column (4 x 50 mm) | 046122 |
| Borate Trap Column | 047078 |
| Amino Trap Column (3 x 30 mm) | 060146 |
For Sensitive Analysis of Mono- and Disaccharides
The Thermo Scientific? CarboPac? PA10 column line has been expanded to include a new 0.4 × 250 mm capillary column. As with all CarboPac PA10 columns, the new capillary format is the ideal choice for mono- and disaccharides in foods, drugs and plants.
The CarboPac PA10 is an ideal choice for separations of mono- and disaccharides found in mammalian glycoproteins. Fucose is well resolved from the system void and from protein hydrolysate interferents. The CarboPac PA10 allows oxygen to elute after mannose, eliminating interference with monosaccharides. It is ideal for the analysis of mono- and disaccharides in foods, drugs, and plants, and separates sialic acids with the addition of sodium acetate to the eluent.
For the quantification of acidic, neutral, and aminomonosaccharides
Simple, isocratic separations
Superior selectivity for common monosaccharides compared to the CarboPac PA1
No derivatization required
Gradient compatible
The CarboPac PA10 is an anion-exchange column designed specifically for high-resolution separations of mono- and disaccharides. Separation is accomplished with a simple isocratic eluent, and detection is accomplished with pulsed amperometric detection (PAD). No derivatization is required.
| CarboPac PA10 Column for Monosaccharide and Disaccharide Analysis Specifications | |
|---|---|
| Dimensions | CarboPac PA10 Analytical Column: 4 × 250 mm, 2 × 250 mm and 0.4 × 250 mm CarboPac PA10 Guard Column: 4 × 50 mm, 2 × 50 mm and 0.4 × 50 mm |
| Maximum operating pressure | 3500 psi (24.5 MPa) |
| Temperature range | 4–55 °C Recommended operating temperature: ambient |
| Typical operating conditions | 1900 psi at 1.0 mL/min (4 mm analytical and guard columns) Recommended flow rate: 1.0 mL/min (4 mm), 0.25 mL/min (2 mm) or 0.010 mL/min (0.4 mm) |
| Mobile phase compatibility | pH 0–14; up to 90% of common HPLC solvents. Avoid anionic detergents and 100% water. Acetate or hydroxide eluents only. |
| Resin composition | 10-μm diameter substrate (ethylvinylbenzene 55% cross-linked with divinylbenzene) agglomerated with 460-nm MicroBead? difunctional quaternary ammonium ion (5% cross-linked). |
| Anion-exchange capacity | Approximately: 100 μeq/column (4 × 250 mm analytical column) 25 μeq/column (2 × 250 mm analytical column) 1 μeq/column (0.4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
