| 概述 |
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| 描述 | beta-Tubulin Mouse Monoclonal Antibody Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells. For example, expression of beta-Tubulin in adipose tissue is very low and therefore beta-Tubulin should not be used as loading control for these tissues. |
| 别名 | β-Tubulin抗体 |
| 宿主 | Mouse |
| 特异性 | beta-Tubulin Mouse Monoclonal antibody detects endogenous levels of total beta-Tubulin protein. |
| 反应种属 | Human;Mouse;Rat;Sheep;Rabbit;Dog;Monkey;Hamster;Chicken |
| 应用 | WB: 1:10000~1:500000, IHC: 1:200, IF/ICC: 1:200, ELISA(peptide) 1:20000-1:40000 |
| 免疫原 | Full-length beta-tubulin protein of human. |
| 性能 |
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| 浓度 | 1mg/ml |
| 纯化方法 | Affinity-chromatography. |
| 类型 | Monoclonal Antibody |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| 存储溶液 | beta-Tubulin Mouse Monoclonal Antibody Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl. |
| 靶标 |
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| 背景说明 | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. |
| 组织特异性 | Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. |
| 翻译后修饰 | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
| 细胞定位 | Cytoskeleton;Extracellular region or secreted;Nucleus; |
| UniProt | Q13509 |
| 实验结果图 |
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| Western blot analysis of A549 (1) , Rat brain (2) ,Mouse brain (3) ,Chicken lung (4) and Rabbit testis (5),Sheep muscle (6) with beta-tubulin mouse mAb. |
| 温馨提示：本产品仅作科研实验使用，不支持临床等研究 |
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