13724130806
当前位置:广州尖分科学仪器有限公司>>戴安 Dionex>>Bio Columns>> MAbPac SEC-1 Monoclonal Antibody Analysis Column
MAbPac SEC– 1 for Monoclonal Antibody (MAb) Analysis
MAbPac™ SEC-1 is a size exclusion chromatography (SEC) column designed for monoclonal antibody (MAb) analysis, including monomers, aggregates, and fragments, under non-denaturing conditions, and in both high- and low-salt phases and volatile eluents.
• Proprietary hydrophilic bonded layer results in minimal non-desired interactions between the biomolecules and the stationary phase.
• Nonmetallic and Biocompatible PEEK™ housing eliminates metal contamination from the column hardware.
• Stable surface bonding leads to low column bleed and compatibility with MS, ELSD and Corona® CAD detection.
• Rugged, reproducible column.
• Superior performance for the analysis of monoclonal antibodies, even at low salt concentrations.
Applications
SEC is frequently used because of its simple operating principle and features not found in other separation techniques including separating aggregates that are not easily distinguished by other chromatographic means. MAbs produced from mammalian cell culture may contain significant amounts of dimers and higher-order aggregates. The MAbPac SEC-10 is a high-resolution SEC column that is suitable for use with a wide range of biomolecules, including monoclonal antibodies and their aggregates.
The MAbPac™ SEC-1 column has a wide molecular operating range as shown in the molecular weight calibration curve.
Protein | MAbPac SEC-1 Retention Time (min) | MW (kDa) |
γ-Globulin aggregate | 7.6 | 7,000 |
Thyroglobulin dimer | 8.0 | 1,338 |
Thyroglobulin | 8.9 | 669 |
γ-Globulin dimer | 10.3 | 300 |
BSA dimer | 11.4 | 134 |
BSA | 12.6 | 67 |
Ovalubumin | 13.3 | 43 |
Trypsin Inhibitor | 14.1 | 22 |
Myoglobin | 14.4 | 18 |
Ribonuclease A | 14.7 | 14 |
Cytochrome C | 14.6 | 12 |
The MAbPac SEC-1 column can be used for quantitative separation of a monoclonal antibody and its aggregates.
Faster MAb size-exclusion separation can be achieved using a shorter column.
请输入账号
请输入密码
请输验证码
以上信息由企业自行提供,信息内容的真实性、准确性和合法性由相关企业负责,化工仪器网对此不承担任何保证责任。
温馨提示:为规避购买风险,建议您在购买产品前务必确认供应商资质及产品质量。