大鼠N-乙酰-β-D-氨基葡萄糖苷酶ELISA Kit

大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA Kit

产品类型:
ELISA Kit

产品名称:

大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA Kit

英文名称:

Rat N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

货号:
大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA Kit别名:
ENC-1AS, N-acetyl-beta-glucosaminidase|hexosaminidase B, Beta-hexosaminidase subunit beta(HEXB)
规格:
96T
中文价格:
大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA Kit种属:
Rat
其他种属:
Human Mouse Pig
待测物名称:
N-acetyl-β-D-glucosaminidase,NAG
缩写:
NAG
检测范围:
1.56 mIU/ml-100 mIU/ml
灵敏度:
0.39 mIU/ml
反应时间:
1-5h
所需样本体积:
50-100ul
检测波长:
450 nm

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated 大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)ELISA Kitantibody specific for NAG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAG bound in the initial step. The color development is stopped and the intensity of the color is measured.