BD Stemflow 人 MSC 分析试剂盒 562245

人多能间充质基质细胞分析试剂盒,BD Stemflow™ Human MSC Analysis Kit

描述：

细胞特性与鉴定标准

人源多能间充质基质细胞(Mesenchymal Stromal Cells, MSCs)，亦称间充质干细胞，是一类具有以下特征的成体干细胞：

• 来源多样性：可从骨髓、脂肪等多种组织中分离获得

• 多向分化潜能：经体外培养可分化为脂肪细胞(adipocytes)、骨细胞(osteocytes)和软骨细胞(chondrocytes)

• 免疫调节功能：在体内外均表现出显著的免疫调控活性

根据国际细胞治疗协会(ISCT)2006年制定的标准，骨髓来源MSCs应满足：

阳性标志物：CD73(+) | CD90(+) | CD105(+)
阴性标志物：CD34(-) | CD45(-) | CD11b/CD14(-) | CD19/CD79α(-) | HLA-DR(-)
其他常见标志物：CD44/CD29/CD200/CD166/CD146/CD271等

试剂盒技术创新

1. 标准化检测方案

• 完整包含ISCT推荐的核心标志物组合

• 阳性混合液(FITC CD90 + PerCP-Cy™5.5 CD105 + APC CD73)

• 阴性混合液(PE CD45/CD34/CD11b/CD19/HLA-DR)

2. 模块化设计优势

• 试剂盒内附PE-CD44抗体

• 其他市售PE标记抗体

• 保留PE通道灵活性，可兼容：

• 支持用户自定义扩展检测项目

3. 实验效率优化

• 多色流式技术降低样本需求量

• 预混抗体组合简化操作流程

• 单独阳性对照抗体便于补偿调节

注：本方案适用于流式细胞术分析，建议配合同型对照设置和荧光补偿对照使用，以获得优良检测结果。

BD Stemflow 人 MSC 分析试剂盒 562245

Human multipotent mesenchymal stromal cells (MSCs), also referred to as mesenchymal stem cells, are a rare population of adult stem cells that can be isolated from a variety of tissues.  MSCs that have been isolated from bone marrow and subsequently cultured can differentiate to a variety of cell types, most notably adipocytes, osteocytes and chondrocytes.  MSCs also have immunomodulatory effects in vivo and in vitro.  In 2006, the International Society for Cellular Therapy (ISCT) proposed a cell surface marker panel for the minimal identification of human MSCs derived from bone marrow.  Under this recommendation MSCs should be positive for CD73, CD90, and CD105, but be negative for CD34, CD45, CD11b or CD14, CD19 or CD79α, and HLA-DR.  MSCs are also known to express numerous cell surface markers such as CD44, CD29, CD200, CD166, CD146 and CD271.  In this kit we include the panel that was proposed by the ISCT.  In addition, we designed this kit to be modular so that additional markers could be "dropped-in".  Specifically, the MSC positive cocktail (FITC CD90, PerCP-Cy™5.5 CD105 and APC CD73) leaves the PE channel open to use in combination with the supplied negative MSC cocktail (PE CD45, PE CD34, PE CD11b, PE CD19 and PE HLA-DR), the included PE CD44 antibody conjugate, or a variety of other commercially available PE antibody conjugates.  Multi color analysis minimizes cell numbers required for an assay and antibody cocktails facilitate a streamlined staining protocol to analyze multiple samples.  Individual positive markers are included for compensation set-up.

准备和存储

在 4°C 下原液保存，避免长时间光照。不要冻结。