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更新时间：2024-01-02 16:17:38浏览次数：673
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| 供货周期 | 现货 | 规格 | T-25 Flask |
|---|---|---|---|
| 货号 | CBP74097 | 应用领域 | 生物产业 |
| 主要用途 | 仅限科研使用 |
ISRE-Luc/NFkB-SEAP/THP-1
| CBP74097 | |
| I. Background | |
THP1 reporter cells are derived from THP-1, a human monocytic cell line that naturally expresses many pattern-recognition receptors, including Toll-like receptors. | |
| II. Description | |
The NF-κB reporter (Luc)-THP-1 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. | |
| III. Introduction | |
| Host Cell: | THP-1 |
| Expressed gene: | ISRE |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | NF-kB reporter, NFkappaB reporter, luciferase reporter, NF-kB reporter, NFkB reporter, nfkb thp1, thp1, NF-kB, nfkb, nf-kb |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+10 µg/ml of blasticidin+ 100 μg/ml of Zeocin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Peripheral blood |
| Disease: | Acute monocytic leukemia |
| Morphology: | Monocyte |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
Figure 1. diABZI on THP1-Dual NFkB-SEAP Activity.
Figure 2. diABZI on THP1-Dual ISRE-Luc Activity. | |
