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上海优宁维生物科技股份有限公司>>公司动态>>小G蛋白研究利器-pull-down or G-LISA试剂盒65折*

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小G蛋白研究利器-pull-down or G-LISA试剂盒65折*

阅读:344        发布时间:2014-5-23

小G蛋白研究利器-- pull-down or G-LISA试剂盒65折*

上海优宁维生物科技有限公司

活动时间:2014年6月1日-7月10日

 

近几年,测定细胞或组织中的小G蛋白的活性已经成为信号转导研究者广泛应用的技术。小G蛋白中的Rho家族包含至少有20个成员,其中zui有特性研究做多的是Rho、Rac、 Rrf 、Cdc42、Ras、Ral 。来自美国的Cytoskeleton公司为小G蛋白活性检测提供了两种方法:pull-down(Affinity bead followed by Western blot format)和G-Lisa(Elisa based format).
 



Choose Your Format 

 G-LISA®Pull-down
Total protein per assay10-50 μg500-2000 μg
Assay time<3 h10-12 h (2 days)
Lysate clarification needed* NoYes
Sample handling96 Samples10 Samples
Quantitative data**YesSemi



Pull-down Activation Assays 

   Pull-down assays utilize affinity beads linked to an effector protein that selectively binds active GTPase followed by quantitation with Western blotting.

 

Pull-down Activation AssaysCat. #Amount
Combo RhoA/Rac1/Cdc42 Activation Assay Biochem KitBK0303 x 10 assays
Arf1 Activation Assay Biochem Kit™BK032-S20 assays
Arf6 Activation Assay Biochem Kit™BK033-S20 assays
Cdc42 Activation Assay Biochem Kit™BK034-S20 assays
BK03450 assays
Rac1 Activation Assay Biochem Kit™BK035-S20 assays
BK03550 assays
RalA Activation Assay Biochem Kit™ BK04050 assays
Ras Activation Assay Biochem Kit™BK008-S20 assays
 BK00850 assays
RhoA Activation Assay Biochem Kit™BK036-S20 assays
BK03650 assays


G-LISA® Activation Assays

G-LISAs use a 96-well plate coated with effector protein that selectively binds the active GTPase followed by quantitation with ELISA techniques.

 

G-LISA Activation AssaysCat. #Amount
G-LISA RhoA Activation Assay Biochem Kit (luminescence format)

BK121

96 assays
G-LISA RhoA Activation Assay Biochem Kit (colorimetric format)BK12496 assays
G-LISA Rac1,2,3 Activation Assay Biochem Kit (colorimetric format)BK12596 assays
G-LISA Rac1 Activation Assay Biochem Kit (luminescence format)BK12696 assays
G-LISA Cdc42 Activation Assay Biochem Kit (colorimetric format)BK12796 assays
G-LISA Rac1 Activation Assay Biochem Kit (Colorimetric Based)BK12896 assays
G-LISA RalA Activation Assay Biochem Kit (Colorimetric Based)BK12996 assays
G-LISA Ras Activation Assay Biochem Kit (Colorimetric Based)BK13196 assays
G-LISA Arf1 Activation Assay Biochem Kit (Absorbance Based)BK13296 assays
G-LISA Arf6 Activation Assay Biochem Kit (Absorbance Based)BK13396 assays

 

上海优宁维生物科技有限公司(www.univ-bio.com)

 上海:/89/99info@univ-bio.com北京:/1441/4210beijing@univ-bio.com 
 广州:/07guangzhou@univ-bio.com天津:tianjin@univ-bio.com 
 湖北/湖南:wuhan@univ-bio.com南京/安徽:nanjing@univ-bio.com 
 杭州:hangzhou@univ-bio.com苏州:sz1688@univ-bio.com 
 济南:jinan@univ-bio.com沈阳:shenyang@univ-bio.com 

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