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小G蛋白研究利器-pull-down or G-LISA试剂盒65折*
小G蛋白研究利器-- pull-down or G-LISA试剂盒65折*
上海优宁维生物科技有限公司
活动时间:2014年6月1日-7月10日
近几年,测定细胞或组织中的小G蛋白的活性已经成为信号转导研究者广泛应用的技术。小G蛋白中的Rho家族包含至少有20个成员,其中zui有特性研究做多的是Rho、Rac、 Rrf 、Cdc42、Ras、Ral 。来自美国的Cytoskeleton公司为小G蛋白活性检测提供了两种方法:pull-down(Affinity bead followed by Western blot format)和G-Lisa(Elisa based format).
Choose Your Format
G-LISA® | Pull-down | |
Total protein per assay | 10-50 μg | 500-2000 μg |
Assay time | <3 h | 10-12 h (2 days) |
Lysate clarification needed* | No | Yes |
Sample handling | 96 Samples | 10 Samples |
Quantitative data** | Yes | Semi |
Pull-down Activation Assays
Pull-down assays utilize affinity beads linked to an effector protein that selectively binds active GTPase followed by quantitation with Western blotting.
Pull-down Activation Assays | Cat. # | Amount |
Combo RhoA/Rac1/Cdc42 Activation Assay Biochem Kit | BK030 | 3 x 10 assays |
Arf1 Activation Assay Biochem Kit™ | BK032-S | 20 assays |
Arf6 Activation Assay Biochem Kit™ | BK033-S | 20 assays |
Cdc42 Activation Assay Biochem Kit™ | BK034-S | 20 assays |
BK034 | 50 assays | |
Rac1 Activation Assay Biochem Kit™ | BK035-S | 20 assays |
BK035 | 50 assays | |
RalA Activation Assay Biochem Kit™ | BK040 | 50 assays |
Ras Activation Assay Biochem Kit™ | BK008-S | 20 assays |
BK008 | 50 assays | |
RhoA Activation Assay Biochem Kit™ | BK036-S | 20 assays |
BK036 | 50 assays |
G-LISA® Activation Assays
G-LISAs use a 96-well plate coated with effector protein that selectively binds the active GTPase followed by quantitation with ELISA techniques.
G-LISA Activation Assays | Cat. # | Amount |
G-LISA RhoA Activation Assay Biochem Kit (luminescence format) | BK121 | 96 assays |
G-LISA RhoA Activation Assay Biochem Kit (colorimetric format) | BK124 | 96 assays |
G-LISA Rac1,2,3 Activation Assay Biochem Kit (colorimetric format) | BK125 | 96 assays |
G-LISA Rac1 Activation Assay Biochem Kit (luminescence format) | BK126 | 96 assays |
G-LISA Cdc42 Activation Assay Biochem Kit (colorimetric format) | BK127 | 96 assays |
G-LISA Rac1 Activation Assay Biochem Kit (Colorimetric Based) | BK128 | 96 assays |
G-LISA RalA Activation Assay Biochem Kit (Colorimetric Based) | BK129 | 96 assays |
G-LISA Ras Activation Assay Biochem Kit (Colorimetric Based) | BK131 | 96 assays |
G-LISA Arf1 Activation Assay Biochem Kit (Absorbance Based) | BK132 | 96 assays |
G-LISA Arf6 Activation Assay Biochem Kit (Absorbance Based) | BK133 | 96 assays |
上海优宁维生物科技有限公司(www.univ-bio.com) |
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