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优宁维之一步法完成IP/COIP/pulldown --Nano-traps
德国ChromoTek公司成立于2008年,致力于开发和销售*的研究产品,ChromoTek的宗旨是让客户花zui少的时间获得zui多的信息和zui高高质量的数据。ChromoTek充分利用了抗体的技术,并且提高其性能,主要的应用有:
Reagents for IP and microscopy
Live cell High-Content Assays (HCA)
Protein-Protein Interaction (PPI) Assays
ChromoTek公司的Nano-Traps是来自于alpaca羊驼的偶联有单价矩阵基质(agarose beads,magnetic particles or multiwell plates)的单结构域的抗体片段(VHHs)。
从细胞到凝胶只要30分钟:
·
· From cell to gel in just 30 minutes: Depending on your experimental setup it takes as less as 10 minutes to qunatitatively bind your fusion protein to our Nano-Traps. Followed by a couple of quick wash steps, you are ready to load your gel.
主要的产品有 GFP-Trap® / RFP-Trap®
产品特色:
ChromoTek公司的Nano-Traps是生化分析荧光融合蛋白的强有力的工具
孵育时间短,5-30min
能够从细胞提取物或所有的细胞器中定量分离融合蛋白及结合(暂时性)因子
低非特异性结合
没有普通抗体的重链和轻链的非特异性结合或污染
产品的应用及应用范围如下:
method | material | application |
pulldown | mammalian cells | Co-IP |
purification | issues/ organisms | Mass spectroscopy |
immunoprecipitation (IP) | bacteria | Enzyme activity measurements |
yeast | BiacoreTM/ affinity measurements | |
plants | ChIP analysis |
产品信息
分类 | 货号 | 品名 | 规格 | |
Nano-Traps | GFP-Trap® | gt-250 | GFP-Trap®protein | 250ug |
gta | GFP-Trap®_A | 20rxns/100rxns/200rxns/400rxns | ||
gtak-20 | GFP-Trap®_A Kit | 20 reactions | ||
gtm-20 | GFP-Trap®_M | 20rxns/100rxns/200rxns/400rxns | ||
gtmk-20 | GFP-Trap®_M Kit | 20 reactions | ||
gtp-96 | GFP-multiTrap | 96 reactions | ||
gtp-480 | GFP-multiTrap | 480 reactions | ||
RFP-Trap® | rt-250 | RFP-Trap®protein | 250ug | |
rta | RFP-Trap®_A | 20rxns/100rxns/200rxns/400rxns | ||
rtak-20 | RFP-Trap®_A Kit | 20 reactions | ||
rtm-20 | RFP-Trap®_M | 20rxns/100rxns/200rxns/400rxns | ||
rtmk-20 | RFP-Trap®_M Kit | 20 reactions | ||
GST-Trap | stm | GST-Trap_M | 20rxns/100rxns/200rxns/400rxns | |
stmk-20 | GST-Trap_M Kit | 20 reactions | ||
st-250 | GST-Trap uncoupled, purified protein | 250ul | ||
Dnmt1-Trap | dt-250 | Dnmt1-Trap uncoupled, purified protein | 250ul | |
dta | Dnmt1-Trap_A | 20rxns/100rxns/200rxns/400rxns | ||
dtak-20 | Dnmt1-Trap_A Kit | 20 reactions | ||
Binding controls | bab-20 | Blocked agarose beads | 20 reactions | |
bmp-20 | Blocked magnetic beads | 20 reactions |
常规抗体
货号 | 品名 | 规格 | |
conventional monoclonal antibodies | 2e8 | Dnmt1 antibody (rat monoclonal) | 100 µl |
4b8 | Ago1 antibody Rat monoclonal | 100ug | |
11a9 | Ago2 antibody Rat monoclonal | 100ug | |
9e1 | c-Myc antibody Rat monoclonal | 100ug | |
2e8 | Dnmt1 antibody Rat monoclonal | 100ug | |
6f7 | FLAG antibody Rat monoclonal | 100ug | |
3h9 | GFP antibody Rat monoclonal | 100 µl | |
6G9 | GST antibody (rat monoclonal) | 100 µl | |
7c9 | HA antibody Rat monoclonal | 100 µl | |
16d10 | PCNA antibody Rat monoclonal | 100 µl | |
3f5 | RFP antibody (mouse monoclonal) | 100 µl | |
5f8 | RFP antibody (rat monoclonal) | 100 µl | |
1c7 | RNA Pol II CTD Antibody Rat monoclonal | 5 ml |
上海优宁维生物科技有限公司
地址:上海浦东新区古丹路15弄16号楼
:4008-168-068
:/89
:info@univ-bio.com
:www.univ-bio.com
我们会尽所能的帮助您。提供*、zui全面的抗体、流式抗体、免疫组化服务