NUCLEOGEL® RP液相色谱柱

NUCLEOGEL® RP

Polymer-based reversed phase HPLC phases
for biochemical applications

Features

Polystyrene resin cross-linked with divinylbenzene, available particle sizes 5 μm and 8 μm,
available pore sizes 100 Å and 300 Å ∙ USP L21
pH working range 1–13, max. working pressure 180 bar
eluent in column acetonitrile – water

Small pore columns for reversed phase separation of small molecules such as pharmaceuticals with basic properties, e.g., organic heterocycles; also suited for separation of nucleosides and nucleotides up to 5000 daltons; allow gradient as well as isocratic elution

Wide pore columns especially recommended for large biomolecules (proteins, peptides etc.),
higher background hydrophobicity compared to silica phases

For more applications click here.

万古霉素vancomycin的定量分析

色谱柱：VA 150×4.6mm NUCLEOGEL RP 100-8
流动相：A:8%(v/v) acetonitrile-0.02M borate buffer PH 8.0
       B:16%(v/v) acetonitrile-0.02M borate buffer PH 8.0  梯度洗脱：0-* B in 17.5min
流  速：0.5毫升/分钟
检测器：UV 235nm

蛋白的分析

色谱柱：VA 50mm×4.6mm  NUCLEOGEL RP 300-8
流动相：A:0.1%TFA 水溶液   梯度洗脱：20-60%   B,22min
流  速：1.5毫升/分钟
检测器：UV 220nm
样  品：1.Ribonuclease  2.Insulin  3.cytochrome c  4.Iysozyme  5.bovine serum albumin6.Myoglobin  7.Ovalbumin

NUCLEOGEL RP C18分析拟交感神经胺

色谱柱：VA 150/4.6 NUCLEOGEL 80-10 C18
流动相：A:0.1M 氢氧化胺：乙腈=68:32  B:0.1M氢氧化胺：乙腈=54:46  梯度洗脱0-* B,5min
流  速：1.0毫升/分钟    进样量：20 ul
温  度：室温             检测器：UV 230nm
样  品：1.Phenylpropanolamine   2.Pseudoephedrine   3.Amphetamine   4.Methamphetamine

Ordering information

Length ->

50 mm

150 mm

250 mm

Guard columns*

Valco type analytical columns      

NUCLEOGEL® RP 100-5 (particle size 5 µm, pore size 100 Å)

4.6 mm ID

719454

719455

719542

NUCLEOGEL® RP 100-8 (particle size 8 µm, pore size 100 Å)

4.6 mm ID

719456

719520

719542

NUCLEOGEL® RP 300-5 (particle size 5 µm, pore size 300 Å)

4.6 mm ID

719459

719542

NUCLEOGEL® RP 300-8 (particle size 8 µm, pore size 300 Å)

4.6 mm ID

719460

719542

* Valco type guard column cartridges measure 5 x 3 mm, require guard column holder B (REF 719539) and are supplied in packs of 2. Columns are supplied in packs of 1.