【文献标题】Proanthocyanidins and β‑Glucan Synergistically Regulate Intestinal Inflammation in Dextran Sulfate Sodium-Induced Colitis Mice

【作者】Pengkui Xia, Ruyi Li, Mianhong Chen et.al

【作者单位】Huazhong Agricultural University（华中农业大学）

【文献中引用产品】

小鼠白介素6(IL-6)ELISA试剂盒

小鼠白介素1β (IL-1β)ELISA试剂盒

小鼠白介素10(IL-10)ELISA试剂盒

小鼠肿瘤坏死因子α(TNF-α)ELISA试剂盒

【关键词】proanthocyanidins, β-glucan, colitis, synergistic anti-inflammatory function

【DOI】doi.org/10.1021/acs.jafc.4c03544

【影响因子(IF)】5.69

【出版期刊】《Journal of Agricultural and Food Chemistry》

【产品原文引用】

Biomarker Analysis. Biomarker levels in the colonic tissueswere analyzed. Briefly, 100 mg of colon or liver tissue samples was weighted and homogenized in a precooled phosphate buffered solution (PBS, 1:9 [w/v]). The homogenate samples were centrifuged at 10,000g for 10 min at 4 °C, and the supernatant was collected. The protein concentration of the supernatant was determined by Bicinchoninic acid detection kits (Biosharp Biotechnology Co., Ltd.,Hefei, China). The contents of interleukin (IL)-6, IL-1β, IL-10, andtumor necrosis factor (TNF)-α in the colon were assayed using enzyme-linked immunosorbent assay (ELISA) kits (Shanghai Huding Biotechnology Co., Ltd., Shanghai, China). 

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