【文献标题】Bacteroides-derived isovaleric acid enhances mucosal immunity by facilitating intestinal IgA response in broilers

【作者】Xinkai Wang, Yifan Huet.al

【作者单位】 华中农业大学（Huazhong Agricultural University）

【文献中引用产品】

鸡免疫球蛋白A(IgA)ELISA试剂盒

鸡免疫球蛋白M(IgM)ELISA试剂盒

【关键词】Bacteroides, Chicken, IgA, Intestinal health, Isovaleric acid, Macrophage

【DOI】doi.org/10.1186/s40104-022-00807-y

【影响因子(IF)】6.17

【出版期刊】《Journal of Animal Science and Biotechnology》

【产品原文引用】Intestinal contents or tissues samples were soaked in PBS (100μL to 10mg sample) and homogenized using homogenizer, then centrifuged at 12,000 ×g for 10min to remove small particles, and the supernatant was collected. Free IgA and IgM concentration were detected using chicken IgA, and IgM enzyme-linked immunosorbent assay (ELISA) quantitation Set (HUDING Biology, 

Shanghai, China).

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