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用于使用猪瘟E2亚单位疫苗的猪场净化猪瘟，鉴别野毒感染抗体和疫苗抗体使用。

For detection of antibodies to the Erns protein of the classical swine fever virus (CSFV)

The pigtype CSFV Erns Ab is a double-antigen ELISA. The microtiter plate is coated with recombinant CSFV Erns antigen. During sample incubation, antibodies to the CSFV Erns protein bind to the immobilized antigen. Unbound material is removed by rinsing. Antibodies bound to the antigen are detected by a horseradish peroxidase (HRP) conjugate. Unbound conjugate is removed by rinsing. A colorimetric reaction is initiated by adding Substrate Solution and stopped after 10 minutes. If antibodies to the CSFV Erns protein are present in the sample, a blue color develops, which turns yellow after the addition of Stop Solution. The optical density (OD) is measured in a spectrophotometer at 450 nm. Measurement at a reference wavelength (620-650 nm) is optional. The OD value correlates to the concentration of the antibodies to the CSFV Erns protein in the sample.

能检测家猪或者野猪的血清和血浆中的猪瘟病毒Erns抗体，配合猪瘟标记疫苗（例如硕腾Suvaxyn  CSFV marker等）执行猪瘟免疫净化计划，区分疫苗免疫猪和病毒感染猪。 

猪瘟e0蛋白抗体检测试剂盒 猪瘟抗体检测检测 
酶标板上面包被了纯化重组猪瘟Erns抗原。样品经过孵育处理后，猪瘟Erns抗体能与包被的抗原特异性结合。洗板去除没结合的物质后，每孔加入酶标记物孵育。洗板后，将直接使用的底物/显色剂溶液加入各孔，如果待测样品中含有Erns抗体，将发生蓝色颜色反应。孵育完毕，加入终止液终止颜色反应并在酶标仪450nm测量。颜色反应的显色强度与样品中猪瘟Erns抗体含量成正比。