美国kernel禽传染性喉气管炎抗体检测试剂盒 

规格 2*96,5*96,10*96 

鸡传染性喉气管炎(Avian Infectious Laryngotracheitis， AILT)是由传染性喉气管炎病毒引起的一种急性、接触性上部呼吸道传染病。其特征是呼吸困难、咳嗽和咳出含有血样的渗出物。剖检时可见喉部、气管粘膜肿胀、出血和糜烂。在病的早期患部细胞可形成核内包涵体。本病1925年在美国*报道后，现已遍及世界许多养鸡地区。本病传播快，死亡率较高，在我国较多地区发生和流行，危害养鸡业的发展。 

Infectious laryngotracheitis (commonly known as ILT) is a viral respiratory disease of chickens centred on the trachea. ILT can cause serious production losses due to a combination of mortality and depressed egg production. This disease can manifest itself in a variety of forms ranging from severe to mild with the latter being increasingly encountered in developed poultry industries and appearing as a mucoid tracheitis, sinusitis and conjunctivitis coupled to unthriftiness and low mortality. 

INTENDED USE 
ELI-LTI ELISA kit is used for quantitative detection of antibodies against Infectious Laryngotracheitis virus (ILT) in chicken serum and yolk. 
Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-ILT antibodies present in the serum will bind to the AIV on the microwell forming an AIV antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the ILT antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 - 410 nm with a 630-650 nm reference filter.