猪瘟病毒抗体检测试剂盒

Classical Swine Fever Virus Antibody Test Kit (CSFV Ab)

概述

猪瘟病毒（CSFV）、牛病毒性腹泻性病毒(BVDV)以及边界病（BDV）都属于黄病毒属的成员。由于CSFV的高致病力和高致死率，它已给养殖业造成了巨大的损失。猪感染高致病力毒株后在潜伏期就可以造成传染，经历急性猪瘟或亚急性猪瘟临床症状的猪在出现症状前大量排毒，但耐过后的猪只体内有明显的CSFV抗体，且不再排毒。感染温和性毒株的猪呈慢性感染，在病死前会长期或间歇性地排毒。怀孕母猪可以通过胎盘感染胎儿导致流产、木乃伊化或产出弱猪、畸形胎等。先天性感染低毒力病毒的结果是产出持续性感染的仔猪，仔猪出现免疫耐受，不表现任何症状向外排毒。猪也有可能感染BVDV或BDV。尽管这些感染通常呈温和过程并且是自限性的，但有效的将它们同猪瘟病毒区分是很重要的。

原理

猪瘟病毒抗体ELISA检测试剂盒是用来检测猪血清或血浆中猪瘟病毒抗体的检测试剂盒。该试剂盒是用猪瘟病毒抗原包被的微量反应板，利用阻断ELISA原理来检测猪血清或血浆中猪瘟病毒抗体。如果被检样品中存在猪瘟病毒抗体，它们就会阻断辣根过氧化物酶标记（HRPO）的抗猪瘟病毒的单克隆抗体。单克隆抗体与猪瘟病毒抗原的结合可以通过辣根过氧化物酶与底物的显色程度进行判定，即用酶标仪在单波长450nm或双波长450nm与620nm测定该反应体系的吸光度。当被检样品中含有猪瘟病毒抗体（阳性结果）时，显色就会变浅，当被检样品中不含有抗猪瘟病毒抗体（阴性结果）时，显色就会变深。样本的阻断率可以通过450nm波长样本吸光度与阴性对照吸光度的比值来确定。

The PrioCHECK? CSFV Antibody 2.0 ELISA is a blocking ELISA that specifically detects antibodies against the A domain of the E2 protein of CSFV. This domain is more specific for CSFV than the B, C, and D domain of the E2 protein. An important advantage of the test is that it does not cross-react with related viruses such as Bovine Viral Diarrhoea virus (BVDV) and Border Disease virus (BDV). The test detects antibodies against high-, moderate-, and low-virulent CSFV strains early after infection. Detection of antibodies elicited by low-virulent CSFV strains is of paramount importance to trace sub clinical CSFV infections.

Test procedure
The PrioCHECK? CSFV Antibody 2.0 ELISA is a blocking ELISA. In this test, the plates are coated with the E2 CSFV envelope protein. The key reagent is a monoclonal antibody (mAb) directed against an epitope located on the A domain of the envelope protein E2 of CSFV, making the test more specific for CSFV. The mAb is labeled with an enzyme that generates a color signal. The signal is measured and when no color is formed, the sample containing anti-CSFV antibodies that competed for the A domain of the E2 protein is positive for CSFV.