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BDP 630/650 X NHS ester
BDP 630/650 X NHS ester
BDP 630/650 is a borondipyrromethene fluorophore that has a high molar extinction coefficient, excellent quantum yield, and a relatively long lifetime of the excited state. Due to it, this fluorophore is useful for fluorescence polarization assays that allow to detect binding between molecules.
This is an amine reactive NHS ester. It contains an aminohexanoyl linker between the fluorophore and the reactive group.
General properties
| Appearance: | dark colored solid |
| Mass spec M+ increment: | 545.2 |
| Molecular weight: | 660.5 |
| CAS number: | 380367-48-6 |
| Molecular formula: | C33H31N4BF2O6S |
| IUPAC name: | Succinimidyl-6-[2-(p-{(E)-2-[4,4-difluoro-5-(2-thienyl)-3a,4a-diaza-4-bora-s-indacen-3-yl]ethenyl}phenoxy)acetylamino]hexanoate |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 628 |
| ε, L⋅mol−1⋅cm−1: | 97000 |
| Emission maximum, nm: | 642 |
| Fluorescence quantum yield: | 0.91 |
| CF260: | 0.029 |
| CF280: | 0.035 |
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