PDPH，MBS，LC-SPDP，GMBS 双功能交联剂

PDPH，MBS，LC-SPDP，GMBS 双功能交联剂

产品名称：GMBS

中文名称：4-马来酰亚胺基丁酸-N-琥珀酰亚胺酯

英文名称：4-Maleimidobutyric acid N-hydroxysuccinimide ester

CAS #: 80307-12-6

简介：GMBS is a non-cleavable heterobifunctional protein crosslinker. The GMBS crosslinking reagent is commonly used to crosslink haptens to carrier proteins and enzymes to antibody. The GMBS has a spacer arm length of 7.3 angstroms and is less immunogenic than the crosslinking analog MBS. The GMBS crosslinking reagent's NHS ester and maleimide reactive groups react with amino and sulfhydryl moites, respectively.

刺激是不可切割的异型蛋白交联剂。的刺激是的交联剂交联的半抗原载体蛋白和抗体。的刺激有7.3埃的间隔臂的长度和较低的原性的化交联。交联剂的刺激的NHS酯和马来酰亚胺基团的活性氨基和巯基moites反应。

Application

A heterobifunctional cross-linking reagent with amine and sulfhydryl reactivity. Typically, coupled initially to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). Second coupling specific for molecules containing free sulfhydryl by thioether linkage buffered at pH 6.8 (6.5-7.0). Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates. Provides a 7-atom linker.

结构式：

Molecular Weight: 280.2

分子式 C12H12N2O6

CAS #: 80307-12-6

Spacer Length: 7.3 Å

Alternative Name: 4-Maleimdobutyric acid N-succinimidyl ester

纯度：98%+

保存：-20℃

：西安瑞禧生物科技有限公司

产品名称：LC-SPDP

英文全称：(succinimidyl 6-[3(2-pyridyldithio)propionamido]hexanoate)

General description

The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents – see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures.

我们试验试剂胺活性部分N-羟基琥珀酰亚胺（NHS）酯。反应通常在磷酸盐、碳酸盐/碳酸氢盐或硼酸盐缓冲液中进行。如果不含伯胺（或硫醇或二硫键还原试剂）-见巯基反应性，可以使用其他缓冲剂。反应速率和水解降解率随pH值的增加而增加；例如，ph值为7的ph值为9小时，pH值小于10分钟。NHS酯试剂SPDP和lc-spdp有限的水溶解度和溶解在有机溶剂中之前，添加到反应混合物。磺NHS酯试剂磺LC SPDP是水溶性的，可以直接加入到反应混合物的水

简介：LC-SPDP crosslinker is a cleavable, water insoluble, amino and thiol (sulfhydryl) reactive het-erobifunctional protein crosslinker. The "long chain" LC-SPDP crosslinking reagent has a 15.7 angstrom spacer arm, compared to the 6.8 angstrom spacer arm of SPDP.

结构式：

外观：白色干燥粉末

Molecular Weight: 425.5

分子式C18H23O5N3S2

溶解性：DMSO or DMF

Spacer Length: 15.7 Å

Alternative Name: Succinimidyl-6-(3-[2-pyridyldithiol-propionamido) hexanoate

纯度：98%+

保存：-20℃

：西安瑞禧生物科技有限公司

产品名称：MBS

英文名称：3-Maleimidobenzoic acid N-hydroxysuccinimide ester

中文名称：3-马来酰亚胺基苯甲酸琥珀酰亚胺酯

CAS #: 58626-38-3

简介：MBS crosslinker is a non-cleavable, water insoluble, heterobifunctional protein crosslinking rea-gent that is commonly used to crosslink haptens to carrier proteins and enzymes to antibody. The MBS crosslinking reagent's NHS ester and maleimide reactive groups react with amino and sulfhydryl moites, respectively.

对伯胺和巯基具有反应性的双异官能化交联剂。通常，初始反应在 pH 为 7.0 时，通过酰胺键偶联含伯胺的分子，然后在相同 pH 通过硫醚偶联含巯基的化合物。用于制备交联剂和半抗原载体分子偶联物。

结构式：

Molecular Weight: 314.3

分子式：C15H10N2O6 

CAS #: 58626-38-3

Spacer Length: 7.3 Å

纯度：98%+

保存：-20℃

：西安瑞禧生物科技有限公司

产品名称：PDPH

英文全称：(3-(2-pyridyldithio)propionyl hydrazide)

简介：PDPH is a cleavable, heterobifunctional protein crosslinking reagent. The PDPH crosslinker's hydrazide group selectively reacts with oxidized carbohydrates (or carboxyls when used with EDC); while the pyridinyldisulfide moiety is reactive toward reduced thiols. Thus, PDPH protein crosslinker is an excellent choice for crosslinking glycoproteins and other polysaccharides.

PDPH是裂解，异型双功能蛋白交联试剂。PDPH的酰肼基团的交联剂的反应选择性氧化碳水化合物（或羧基与使用EDC）；而pyridinyldisulfide部分活性对降低硫醇。因此，蛋白质交联剂交联PDPH的糖蛋白和多糖等的一个的选择。

General description

The PDPH is a heterobifunctional crosslinker containing sulfhydryl-reactive pyridyldithiol and carbonyl-reactive hydrazide moieties. Pyridyldithiols react with free sulfhydryls (-SH) to form disulfide bonds. Hydrazide groups react with carbonyls (aldehydes and ketones) to form stable hydrazone bonds. Aldehyde groups can be created by periodate-oxidation of sialic acid and other sugar components of glycoprotein polysaccharides. Thus, PDPH is useful for conjugating glycoproteins and sulfhydryl-containing peptides or proteins. Likewise, PDPH is useful as a sulfhydryl-addition reagent for glycoproteins and other carbohydrates; after reaction of the hydrazide to an oxidized carbohydrate, the pyridyldithiol group can be cleaved by a reducing agent to expose a sulfhydryl group. Yet another application for PDPH is to react the primary amine of the hydrazide moiety to a carboxyl group using the crosslinker EDC.

结构式：

外观：白色粉末   

Molecular Weight: 229.3

分子式：C8N3S2OH11 

溶解性：DMSO or DMF

Spacer Length: 9.2 Å

Alternative Name: 3-[2-Pyridyldithio]propionyl hydrazide

纯度：98%+

保存：-20℃

：西安瑞禧生物科技有限公司

Features and Benefits

• Reactive groups: pyridyldisulfide and hydrazide

• Reactive toward: sulfhydryl groups and carbonyl (aldehyde) groups

• Short (9.2A), sulfhydryl-to-aldehyde crosslinker with disulfide bond spacer arm (cleavable)

• Pyridyldithiol group results in attachment to sulfhydryls via disulfide bond, which can be cleaved with DTT, TCEP or other reducing agents

• Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates

• Use sodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups

• Use with EDC to conjugate primary amine of hydrazide group to carboxyl groups

产品名称：SBA

英文名称：Bromoacetic Acid N-Succinimidyl Ester

中文名称：溴乙酸-N-琥珀酰亚胺酯

CAS #: 42014-51-7

简介：SBA crosslinker (N-Succinimidyl bromoacetate) is a sulfhydryl (thiol) and amino reactive hetero-bifunctional protein crosslinking reagent. SBA crosslinker is non-cleavable and is among the shortest amine and sulfhydryl reactive crosslinking reagents known with a spacer arm length of only 1.5 Angstroms.

该交联剂（溴酯）是一种巯基（硫醇）和氨基反应异型双功能蛋白交联试剂。该交联剂是非裂解和在短的氨基和巯基交联试剂与一个只有1.5埃的间隔臂的长度已知。

A heterobifunctional cross-linking reagent which allows bromoacetylation of primary amine groups followed by coupling to sulfhydryl-containing compounds. Typically, initial reaction couples via ester to primary amine by amide bond formation in the pH range 6.5-8.5. The second reaction results in thioether bonding in pH range 7.0-8.0.

外观：白色粉末

Molecular Weight: 236.02

分子式:C6H6BrNO4

CAS #: 42014-51-7

Spacer Length: 1.5 Å

Alternative Names: Bromoacetic acid N-hydroxysuccinimide ester;

纯度：98%+

保存：-20℃

：西安瑞禧生物科技有限公司

产品名称：SIA

英文名称：N-Succinimidyl Iodoacetate

中文名称：碘乙酸 N-羟基琥珀酰亚胺酯

CAS #: 39028-27-8

简介：N-Succinimidyl iodoacetate (SIA) is a non-cleavable, heterobifunctional protein crosslinker. SIA is among the shortest amine and sulfhydryl (thiol) reactive crosslinkers known with a spacer arm length of 1.5 Angstroms.

具有氨基和巯基反应性的双异官能团交联剂。情况下，初通过 pH 7.5 (6.5-8.5) 缓冲条件下的酰胺键偶联到含有伯胺的分子上，接着通过 pH 7.5 (7.0-8.0) 缓冲条件下的硫醚链与含有自由巯基的分子进行特异性次级键合。用于制备交联剂和半抗原载体分子偶联物。提供两个原子的连接基。

外观：白色粉末

Molecular Weight: 283.02

分子式:C6H6INO4

溶解性：DMF

CAS #: 39028-27-8

Spacer Length: 1.5 Å

Alternative Names: Iodoacetic acid N-hydroxysuccinimide ester; Succinimidyl iodoacetate

纯度：98%+

保存：-20℃

：西安瑞禧生物科技有限公司