24 roundwell microplates 24个圆孔微孔板

24 roundwell microplates 24个圆孔微孔板

24 roundwell microplates 24个圆孔微孔板

24个圆井微孔板（例如康宁公司生产的）CHO细胞的标准条件：•0.75-1毫升培养液•在225转/分时的50毫米震动幅度，或在300转/分时的25毫米震动幅度•三明治盖CR1524a或CR1324a•夹钳CR1800（可堆叠三个板+三明治盖，（见下面的照片）进一步的细节：这种平板格式已经被用于CHO细胞很多年了，从CHO细胞系没有现在那么健壮开始。在伦敦大学学院和剑桥医学院（英国）的一个合作项目中，从康宁和我们的三明治盖和夹钳（Silk等人，2010年，《生物技术快报》，32:73-78）中验证并优化了CHO细胞的培养，轨道振动产生温和的混合（非常低的剪切应力），因此即使是脆弱的动物细胞系也可以在这些板中培养。这些板的另一个优点（与上面所述的正方形深孔微滴定板相比）是由两个壁而不是一个壁分开的。进一步降低了交叉污染的可能性。此外，康宁公司生产的24个圆孔板在聚苯乙烯与蛋白质的结合特性方面具有不同的质量（TC处理、超低附着、未处理等）

24 roundwell microplates (e.g. from Corning)Standard conditions for CHO-cells:•0.75-1 ml culture volumes •50 mm shaking amplitude at 225 rpm, or •25 mm shaking amplitude at 300 rpm •sandwich covers CR1524a or CR1324a •clamp CR1800 (three plates + sandwich covers can be stacked, see photo below)Further details:This plate format has been used already for many years for CHO-cells, starting in the time that CHO-cell lines were less robust than that they are at present. In a collaborative project of the UCL in London and Medimmune in Cambridge (UK), the c*tion of CHO-cells was validated and optimized in these plates from Corning and our sandwich covers and clamps (Silk et al, 2010, Biotechnology Letters, 32:73–78).Because of the round well format, orbital shaking gives rise to a gentle mixing (very low shear stress), and so even the most vulnerable animal cell lines can be c*ted in these plates.Another advantage of these plates (in comparison to the square-deepwell microtiter plates as described above) is that the cultures are separated by two walls rather than one, which further reduces the chance of cross-contamination.Furthermore, the 24-roundwell plates from Corning are available in various qualities in terms of binding characteristics of the polystyrene for proteins (TC-treated, ultra-low attachment, untreated, etc.)

 注：杨清辰发布