24-square deepwell plates 24平方深孔板

24-square deepwell plates 24平方深孔板

24-square deepwell plates 24平方深孔板

24平方深孔板（用于文库相对较小的克隆选择，以及更详细地研究所选克隆）CHO细胞的标准条件：•2.5-3.5毫升培养体积•225转/分时50毫米振荡幅度，或300转/分时25毫米振荡幅度•三明治盖CR1224a，或CR1224c•夹钳CR1700（能够快速取出和放回MTPs+封盖），或•夹钳CR1800（多用途夹钳，如果也使用浅孔板，则为理想选择）更多细节：使用24平方深孔微型板培养CHO细胞比使用96平方深孔板（如上所述）重要得多。50毫米/225转/分和25毫米/300转/分对2.5至3.5毫升的培养量都有很好的效果。据报道，振幅为12毫米的摇瓶器也有很好的效果（如果以大约500转/分的速度运行）。振幅为3毫米的摇瓶器不能用于24平方深的平板：培养液不能跟随摇瓶器的运动，因此会出现所谓的异相条件（见右图），因为培养体积较大，结果通常是可重复的，当然也包括细胞的数量它们的产物（如抗体）更大。因此，这种平板格式通常用于对初选择的96孔平板格式的克隆进行更详细的研究。如果每隔几天培养一次，使用大2.5毫升培养体积。在较大的培养体积下，培养物离盖子太近（甚至可能与盖子直接接触），并且在喂食过程中（在取下三明治盖子，并再次将其放回原处）更容易发生交叉污染。

 24-square deepwell plates  (for clone selection with relatively small libraries, and for studying selected clones iin more detail)Standard conditions for CHO-cells:•2.5-3.5 ml culture volumes •50 mm shaking amplitude at 225 rpm, or •25 mm shaking amplitude at 300 rpm •sandwich covers CR1224a, or CR1224c •clamps CR1700 (enabling quickly taking out and putting back MTPs + covers), or •clamp CR1800 (versatile clamp, ideal if also shallow well plates are used)Further details: The use of 24-square deepwell microplates for the c*tion of CHO-cells is much less critical than the use of 96-square deepwell plates as described above. Both 50 mm / 225 rpm, and 25 mm / 300 rpm give good results for culture volumes between 2.5 and 3.5 ml. Also shakers with an amplitude of 12 mm have been reported to give good results (if run at approx 500 rpm). Shakers with an amplitude of 3 mm - however - can not be used for 24 square deepwell plates: the culture fluid can not follow the movement of the shaker, and so called out-of-phase conditions will occur (see photo on the right)Because of the higher culture volume, the results are generally well reproducible, and of course also the amounts of cells and their products (such as antibodies) are larger. Therefore, this plate format is often used for more detailed studies on clones that were initially selected in the 96-well plate format. If the cultures are to be fed every few days, it is advisable to use maximally 2.5 ml culture volume. At larger culture volumes, the culture is too close to the cover (and may even get in direct touch with the cover), and cross-contamination is more likely to occur during the feeding procedure (during taking of the sandwich cover, and putting it back on again).

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