Gastight box 气密箱 CR1601

Gastight box 气密箱 CR1601

Gastight box 气密箱 CR1601

气密箱CR1601（内夹）气密箱内夹（CR1601）使需要特定气体环境（如较高的二氧化碳浓度或无活力培养）的微量平板发酵成为可能。内部夹持系统与lowwell MTPs的夹持系统相同（见lowwell板盖和夹持器），但该系统也可容纳带夹层盖的深井板。气密箱可安装在Kuhner的振动台上，或在振动台上增加一些额外的孔后，安装在所有其他类型的振动台上，其结构能够承受高达300转/分的持续振动，振幅为25毫米，225转/分的持续振动，振幅为50毫米。由于轨道振动条件的这些限制，我们建议使用24口井MTP和井盖，或者使用方形半深井MTP和96口井MTP（CR 1596b）的通用井盖。气密箱有一个进气口和一个排气口（均通过球阀），与标准的6毫米推入式连接器兼容。盒子气密（在有机玻璃盒子和碳接地板之间使用三元乙丙橡胶密封），但不耐超压或超压超过0.1巴。因此，不能用真空循环使箱体厌氧，必须进行冲洗。尺寸（接地板220x194 mm，总高度包括球阀220mm）允许进出大多数通风室（通过气闸室：只要阀门打开，就可以使用真空循环，或将有机玻璃箱和铝基板分别移入厌氧室，用聚四氟乙烯管（外径6mm）在摇动过程中进行连续冲洗，总重量（包括微量滴定板和盖）约为2.5kg，这意味着，也由于相对较高的重量点，多可将4-6个箱子安装在一个振动台上（取决于振动台型号）。气密箱可容纳两个带夹层盖的深井MTP，或六个带夹层盖的低井MTP。

 Gastight box CR1601 (with clamp inside) The gastight box with a clamp inside (CR1601) enables microtiter plate fermentations requiring a specific gas-atmosphere (e.g. a higher CO2 concentration, or anearobic c*tions). The clamp system inside is identical to the clamp system for lowwell MTPs (see Lowwell plate covers and clamps), but this system can also harbour deepwell plates with sandwich covers.The gastight box can be mounted onto shaking platforms from Kuhner, or - after adding some extra holes in the shaker platform - on all other shaker types, and has been constructed to withstand  continuous shaking up to 300 rpm at an amplitude of 25 mm, and 225 rpm at an amplitude of 50 mm. Because of these limitations in orbital shaking conditions, we advise to work either with 24 well MTPs and covers or with square half deepwell MTPs in combination with our universal covers for 96-well MTPs (CR 1596b)The gastight box has a gas inlet and a gas outlet (both via a ball-valve), that is compatible with standard 6 mm push-in connectors.The box is gastight (using an EPDM rubber sealing between the perspex box and the carbon groundplate), but is not resistant to overpressure or underpressure more than 0.1 bar. Therefore, the box can not be made anaerobic using vacuum cycles, but has to be flushed instead. The dimensions (groundplate 220x194 mm, total height including ball valves 220mm) allow the transfer in and out most anearobic chambers (via an airlock: vacuum cycles can be used provided the valves of the box are open, or the perspex box and the alu groundplate are transferred into the anaerobic chamber separately).Continuous flushing during shaking is feasible using PTFE tubing (outside diameter 6 mm).The total weight (including microtiter plates and covers) is approximately 2.5 kg, which implies that - also due to the relatively high heavy point - maximally 4-6 boxes can be fitted onto one shaking platform (depending on the shaker model).The gastight box can harbour either two deepwell MTPs with sandwich covers, or six lowwell MTPs with sandwich covers.

 注：杨清辰发布