96-roundwell microplate 300 rpm

96-roundwell microplate 300 rpm

96-roundwell microplate 300 rpm

96圆井微板孔内流体力学

对于96个圆孔板，使用0.1至0.2毫升的培养体积，在50毫米振幅的振动筛上以300转/分的速度进行培养。这种振动条件导致氧传输速率为20-40mmol/O2/L/h（对应于100-200 H-1的KLA值）。对于良好的混合，我们建议使用不超过0.15毫升培养体积（或者如果需要更高的氧传输速率甚至更少）。如此低的培养体积使离心后的上清液很难取出。一个更容易做到这一点的技巧是在离心之前加入等量的水或缓冲液（例如0.15ml ul）。请注意，在摇动条件下，你几乎没有空间进行操作：需要小2.5g的离心力（如50 mm/300 rpm）来克服表面张力，这样就可以*混合到底部，防止“死区”。

50毫米振动筛振幅•300转/分

Hydrodynamics inside wells of a 96-roundwell microplate
For 96-roundwell plates, culture volumes between 0.1  and 0.2  ml are used, preferably at 300 rpm on a shaker with a 50 mm amplitude. This shaking condition leads to oxygen-transfer rates of 20-40 mmol O2/L/h (corresponding to kLa values of 100-200 h-1).For a good mixing, we advice to use no more than 0.15 ml culture volume (or even less if higher oxygen-transfer rates are needed). Such low culture volumes make it difficult to take off the supernatant after centrifugation. A trick to make this easier is to add an equal volume (so e.g. 0.15 ml ul) of water or buffer prior to centrifugation.Please note that you have little room to manouvre with regard to the shaking conditions: a centrifugal force in the order of minimally 2.5 g (as with 50 mm/300 rpm) is needed to overcome the surface tension, and so to obtain full mixing down to the bottom of the culture, and prevent “ dead zones”
50 mm shaker amplitude•300 rpm

 注：杨清辰发布