96-square deepwell microplate 300rpm

96-square deepwell microplate 300rpm

96-square deepwell microplate 300rpm

96平方深井微板孔内流体力学

对于96平方深孔微量滴定板，通常使用0.5毫升至0.75毫升的培养体积，在50毫米振幅的振动筛上以300转/分的速度进行培养（右图为照片和视频）。对于0.5毫升的培养物，这些振动条件导致38 mmol O2 l-1 h-1的氧转移速率（OTR）（对应于200 h-1的kLa值）。这样的OTR在其转弯中对应于在ErnMEMEER振动筛烧瓶中的可实现（总瓶体积的培养体积10%，300转/分），并且进一步确保混合到底部（无死区）。后者特别适用于大而重的细胞（如酵母菌和CHO细胞），这些细胞往往在死区沉淀。有些组使用0.6毫升的培养液，而不是0.5毫升，这仍然导致可接受的混合程度。

50毫米振动幅度：300转/分钟（*混合至0.5毫升培养体积）

Hydrodynamics inside wells of a 96-square deepwell microplate
For 96-square deepwell microtiter plates, culture volumes between 0.5 ml and  0.75 ml are typically used, preferably at 300 rpm on a shaker with a 50 mm amplitude (photo and video on the right).For 0.5 ml cultures, these shaking conditions lead to oxygen transfer rates (OTR) of 38 mmol O2 l-1 h-1  (corresponding to kLa values of 200 h-1). Such an OTR - in its turn - corresponds to the high end achievable in Erlenmeyer shaker flasks (culture volume 10% of total flask volume, 300 rpm), and furthermore ensures mixing down to the bottom (no dead zones). The latter is especially relevant  for large and heavy cells (such as yeasts and CHO-cells) that tend to precipitate in dead zones. Some groups use culture volumes of 0.6 ml instead of 0.5 ml, which still results in an acceptable degree of mixing.
50 mm shaker amplitude•300 rpm(full mixing up to 0.5 ml culture volume)

 注：杨清辰发布