96-square half deepwell microplate 300

96-square half deepwell microplate 300

96-square half deepwell microplate 300

96方半深孔微板孔内流体力学

对于96平方的半深孔微量滴定板（CR1496c，专门用于培养目的），通常使用0.25-0.35毫升的培养体积，在50毫米振幅的振动筛上。振荡频率取决于培养液的体积，对于0.25或0.3ml的培养液，一般采用225或250转/分，这种微孔板的氧传递速率（OTR）尚未测定。从96平方深井板（Duetz等人，2000，应用和环境微生物学66:2641-2646）获得的数据推断，在225转/分、40 mmol O2 l-1 h-1（对应于200 h-1的kLa值）下，估计的OTR为250 ul。这样的OTR对应于在ErnMeYER振动筛烧瓶中的可实现（总瓶体积的培养体积10%，300转/分），以确保*混合到底部（无死区），然而，建议使用250转/分的振荡频率而不是225转/分。在不使用高于0.25毫升的培养液的情况下（见本页底部的两个视频蒸馏器。如果只有25毫米振幅的振动筛可用，则可以在培养液不接触盖子的情况下使用更高的振动频率（275或300转/分），还报告了在300转/分（培养体积为0.25毫升）下摇动以获得良好的结果（流体力学和氧转移速率与500毫米/225转/分相当）

建议（默认）1∶25毫米振动筛振幅，300转/分钟（大肠杆菌培养NB，20倍减慢）0.25毫升0.3毫升0.35毫升（*混合至0.25毫升培养体积）

Hydrodynamics inside wells of a 96-square half deepwell microplate
For 96-square half deepwell microtiter plates (CR1496c, specifically designed for c*tion purposes), culture volumes between 0.25 ml and  0.35 ml are typically used, preferably on a shaker with a 50 mm amplitude. The optimal shaking frequency depends on the culture volume.For 0.25  or 0.3 ml cultures, 225 or 250 rpm is generally used.Oxygen transfer rates (OTR) for this kind of microplate have not been measured yet. Extrapolation from the data obtained with 96-square deepwell plates (Duetz et al., 2000, Applied and Environmental Microbiology 66:2641-2646) leads to an estimated OTR at 250 ul at 225 rpm of 40 mmol O2 l-1 h-1  (corresponding to kLa values of 200 h-1). Such an OTR corresponds to the high end achievable in Erlenmeyer shaker flasks (culture volume 10% of total flask volume, 300 rpm), To ensure full mixing down to the bottom (no dead zones), however, it may be advisable to use shaking frequencies of 250 rpm instead of 225 rpm. Even higher shaking frequencies (275 or 300 rpm) may be applied without the culture fluid touching upon the cover, on the condition that no higher culture volumes than 0.25 ml are used (see the bottom two video stills on this page.If only a shaker with a 25 mm amplitude is avaialble, shaking at 300 rpm (and a culture volume of 0.25 ml) is also reported to give good results (hydrodynamics and oxygen transfer rates comparable with 500 mm/225 rpm)
Adviced (default) condtion 1:25 mm shaker amplitude, 300 rpm(E. coli culture on NB, 20 times slowed down)    0.25 ml       0.3 ml      0.35 ml   (full mixing up to 0.25 ml culture volume)

 注：杨清辰发布