24-roundwell microplate 300 rpm

24-roundwell microplate 300 rpm

24-roundwell microplate 300 rpm

24圆孔微板孔内流体力学

对于24个圆孔微量滴定板，使用0.5至1毫升的培养体积，大多数情况下，在25毫米振幅的振动筛上以300转/分（视频在左侧）或在50毫米振幅的振动筛上以225转/分（视频在右侧）进行培养。在两种情况下（培养体积为0.75毫升），氧迁移率约为30毫摩尔O2 L-1 H-1（对应于150 H-1的KLA值）。对于以下（放慢）的高速视频，我们使用来自康宁的24个孔板，其广泛用于细菌和细胞的培养；也因为它们在世界各地都有不同的表面处理（导致不同的结合特性）和伽玛辐照。

建议（默认）条件：1:25 mm摇床振幅，300转/分（在NB上培养大肠杆菌，30次减速）0.5毫升0.75毫升1毫升

Hydrodynamics inside wells of a 24-roundwell microplate
For 24-roundwell microtiter plates, culture volumes between 0.5  and 1 ml are used, mostly either at 300 rpm on a shaker with a 25 mm amplitude (video on the left) or at 225 rpm on a shaker with a 50 mm amplitude (video on the right).Under both conditions (at a culture volume of 0.75 ml), oxygen-transfer rates approximate 30 mmol O2 l-1 h-1  (corresponding to kLa values of 150 h-1).For the (slowed-down) high-speed videos below we used 24-well plates from Corning, which are widely used for the c*tion of bacteria and cells, also because they are worldwide available with various surface treatements (causing different binding characteristics) and gamma irradiated.
Adviced (default) condtion 1:25 mm shaker amplitude, 300 rpm(E. coli culture on NB, 30 times slowed down)    0.5 ml       0.75 ml      1 ml

 注：杨清辰发布