24-square deepwell microplate 225 rpm

24-square deepwell microplate 225 rpm

24-square deepwell microplate 225 rpm

4平方深井微板孔内流体力学

对于24个方形深孔微量滴定板，通常使用2.5-3.5毫升的培养体积**，在摇动器上转速为300毫米（左侧为照片和视频）或在50毫米振幅的振动筛上（在右边的照片和视频上为225转/分钟）。在这两种条件下，2.5毫升培养物的氧传输速率约为35-39毫摩尔O2 L-1 H-1（对应于180～200 H-1的K La值）。*优选使用培养体积。2.5毫升，特别是当三明治盖必须偶尔取下时（例如用于取样或喂食）

50毫米振动筛振幅•225转/分

Hydrodynamics inside wells of a 24-square deepwell microplate
For 24-square deepwell microtiter plates, culture volumes between 2.5 ml and 3.5 ml are generally used**, either at 300 rpm on a shaker with a 25 mm amplitude (photo and video on the left) or at 225 rpm on a shaker with a 50 mm amplitude (photo and video on the right).Under both conditions oxygen transfer rates for 2.5 ml cultures are approximately 35-39 mmol O2 l-1 h-1  (corresponding to k La values of 180-200 h-1).** preferably use a culture volume of 2.5 ml, especially when the sandwich cover has to be take off occasionally (e.g. for sampling or feeding)
50 mm shaker amplitude•225 rpm

 注：杨清辰发布