Glass Bottom Dishes玻璃底皿与培养皿

Glass Bottom Dishes玻璃底皿与培养皿

Glass Bottom Dishes玻璃底皿与培养皿

玻璃底皿与培养皿

采用玻璃底皿，工作距离短，荧光、共焦、高N.A.物镜

以及图像分析实验。

中间有缩小的光学窗口（小于15或11毫米）的加热元件将提供更好的

您的样品温度稳定，非常适合与玻璃底盘和长距离工作

目标。但是，如果使用浸入式物镜，这个小窗口将限制进入

整个底面的大型浸没物镜。在这种情况下，加热元件为35mm

应使用间隙/窗TC-E35。使用物镜仍能提供温度稳定性

加热器。

与玻璃底皿相比，玻璃底皿具有良好的热接触性，因为玻璃底为fla，

标准的塑料培养皿通常在底部边缘有一个突出的边缘。这个

边缘把盘子举过受热面。金属垫片/垫圈可用于消除这种气隙，

然而。这将减少浸入式光学元件到盘底部的通道，因此TC-E35x15/11可以

仅用于远程目标。如果使用浸入式光学元件和大型物镜，35毫米加热

元件TC-E35应与目标加热器结合使用。请注意，一些玻璃底

碟子，例如马特克碟子，是由标准的康宁（或猎鹰碟子）制成的。

由于不同品牌的菜有不同的外径，有时大于38毫米，我们做

减少插入件以提供更好的fi和与加热元件内部切口的热接触，

38毫米。我们还制作了一个50毫米的插件，将培养箱和更大的加热元件内的培养皿居中，

里面有50毫米的切口。

图示为安装在磁性显微镜适配器内的加热元件TC-E35/15/11

Glass Bottom Dishes vs. Petri Dishes
Glass bottom dishes are used with short working distance, high N.A. objective in fluorescence, confocal

and image analysis experiments.
The heating elements with reduced optical window in the middle (down to 15 or 11mm) will provide better
temperature stability for your sample, and are perfect for use with glass bottom dishes and long-working-distance
objectives. If immersion objectives are used, however, this small window will limit access
of the large immersion objective to the whole bottom surface. In this case a heating element with 35mm
clearance/window TC-E35 should be used. Temperature stability still can be provided by using an objective
heater.
In contrasts to glass bottom dishes, which provide good thermal contact because of fla glass bottom,
standard plastic Petri dishes very often have a protruding rim alone the bottom edges of the dish. This
rim elevates the dish above heating surface. Metal shims/washes can be used to eliminate this air gap,
however. This will reduce access for the immersion optics to the bottom of the dish, so TC-E35x15/11 can
be used with long-distance objectives only. If using immersion optics and large objectives, 35mm heating
element TC-E35 should be used, combined with an objective heater. Please note, that some glass bottom
dishes, Mattek dishes for example, are fabricated from standard Corning (or Falcon dishes).
Since different brands of dishes have different outside diameter, sometimes larger than 38mm, we make
reducing inserts to provide better fi and thermal contact to the inside cutout of the heating elements,
which is 38mm. We also make a 50mm insert to center the dishes inside incubators and larger heating elements,
which have 50mm inside cutout.
Shown here is a heating element TC-E35/15/11 mounted inside a magnetic microscope adapter

 注：杨清辰发布