Low Profile Perfusion Set 低剖面灌注装置

Low Profile Perfusion Set 低剖面灌注装置

Low Profile Perfusion Set 低剖面灌注装置

用于培养皿的低剖面灌注装置配有PDI插件和50自粘性

生物相容性垫圈，允许使用常规35毫米培养皿组装。

在35mm培养皿中培养的细胞是一种广泛应用的研究工具，包括

膜片钳和细胞内离子探针成像。然而，真正的灌注（持续流入和ou-

解决方案的流）可能很难配置。无外流的药物输送需要一种spri型

微注射器，但终导致污染整个盘子后，只有少数应用。这个

迫使科学家将细胞贴在盖玻片上，放入专门设计的灌注室。

然而，这种转移是一个耗费时间的过程，它会带来潜在的污染和

额外费用。

PDI室是科学家经过几年的膜片钳研究和

培养单细胞体外灌流培养。这个小室有单独的开口

倾倒工作舱内液位波动并预防的进出口

气泡进入腔室。层流轮廓有助于灌注，并提供更快的解决方案

交换。一个可调节的金属吸管（包括）控制着碟子里的液体水平。密室

设计用于灌注系统和带微型球头的磁性支架，可以

调节灌注管。可与玻璃底皿配合使用成像。事实上，一些玻璃杯

底皿由标准培养皿制成，例如康宁35毫米。可与

温度控制系统。吸入管需要连接到流出源CFPS-1U

包括三个磁性管/电极支架和不锈钢吸入管。显微镜适配器MA

是必需的。可与磁性和非磁性显微镜适配器一起使用。如果与非磁性材料一起使用

适配器，需要一套IMA-MH微型支架。项目#：LPPCP1

培养皿插入物

目录号说明

用于培养皿的PDI低剖面室插入物

用于培养皿的LPPCP1低剖面灌注装置

TC-E35x15加热元件，用于孔径为15mm的35mm盘子

TC-E35x11加热元件，适用于孔径为11mm的35mm盘子

TC-PA-C培养皿适配器，用于康宁和马特克培养皿

TC-PA-N培养皿适配器，用于Nunc培养皿

TC-PA-W培养皿适配器，用于Willco培养皿

TC-PA-F培养皿适配器，用于氟化物培养皿

TC-PA-G培养皿适配器，用于Greiner Bio One培养皿，玻璃底

 Low Profile Perfusion Set for Petri dishe Comes with PDI insert and 50 selfadhesive
biocompatible gaskets, which allow to use the assembly with regular 35 mm petri dishes.
Cells cultured in 35 mm Petri dishes are a popular research tool used in numerous applications, including
patch clamping and intracellular ion probe imaging. However, true perfusion (continuous inflow and ou -
flow) of solutions can be difficult to configure. Drug delivery without an outflow requires a spri -type
microinjector, but ultimately results in contamination of the entire dish after only a few applications. This
forces scientists to plate cells on cover slips for placement into specially designed perfusion chambers.
However, such transfer is a time consuming process which introduces the potential for contamination plus
additional expense.
The PDI chamber was designed by scientists after several years of patch clamp research combined with
external perfusion of single cells cultured in Petri dishes. The chamber has separate openings for solution
inflow and outflow that dump the fluctuations of the liquid level in the working compartment and preven
bubbles from entering the chamber. The laminar profile facilitates perfusion and provides faster solution
exchange. An adjustable metal suction tube (included) controls the level of liquid in the dish. The chamber
was designed for use with perfusion systems and magnetic holder with miniature ball-joint, which can
accommodate perfusion manifolds. Can be used with glass bottom dishes for imaging. In fact, some glass
bottom dishes are made from standard petri dishes like Corning 35 mm, for example. Can be used with
temperature controlled systems. The suction tubing requires connection to an outflow source CFPS-1U
Includes three magnetic tubing/electrode holders, and stainless suction tubing. A microscope adapter MA
is required. Can be used with both magnetic and non-magnetic microscope adapters. If used with nonmagnetic
adapters, a IMA-MH set of miniature holders is required. Item#: LPPCP1
Petri Dish Inserts
Catalog No. Description
PDI Low Profile Chamber-Insert for Petri Dishes
LPPCP1 Low Profile Perfusion Set for Petri Dishes
TC-E35x15 Heating Element for 35mm dishes with 15mm aperture
TC-E35x11 Heating Element for 35mm dishes with 11mm aperture
TC-PA-C Petri Dish Adapters, for Corning and Mattek petri dishes
TC-PA-N Petri Dish Adapters, for Nunc for Nunc dishes
TC-PA-W Petri Dish Adapters, for Willco dishes
TC-PA-F Petri Dish Adapters, for FluoroDishes
TC-PA-G Petri Dish Adapter, for Greiner Bio-One dishes, glass bottom

 注：杨清辰发布