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详细介绍
Organ-on-a-Chip片上器官完整设置
说明
一个理想的芯片上的器官应该易于使用，并且可以可逆地访问芯片中的细胞进行下游分析。因此，我们的芯片器官满足所有这些要求有小包装（1个芯片-1个夹子-4个补丁）或大包装（4个芯片-1个夹子-16个补丁）。
该微流控平台由三部分组成：
微流控芯片
夹紧
文化补丁
模块化微流控芯片由两块PC（聚碳酸酯）塑料板组成，每一块都有四块可逆组装用集成磁铁和一个弹性PDMS薄膜，以确保在8千帕压力下良好密封。芯片上部的luer连接器允许药物筛选和/或细胞分化实验期间的介质灌注和动态流动条件。
包括在组件中的卡箍确保了高流量压力（高达100千帕）下的良好密封。该芯片可以放置在一个手动螺纹夹钳中进行组装和快速操作，并且在动态流动条件下的实验结束时，可以通过拆卸夹钳和微流控芯片轻松地移除组织补片。
培养贴片是纳米纤维的单层，支持功能性三维微组织中细胞的组织培养贴片包括在设置中，纳米纤维可以由两种不同的天然聚合物制成，以模拟细胞外基质：
明胶
胶原蛋白
请联系我们，如果您想要一个由合成生物相容性聚合物制成的定制培养贴片：
聚乳酸-乙醇酸共聚物
聚己内酯
聚二甲基戊二酰亚胺
因此，可以使用不同的贴片在培养皿或微孔板中培养细胞或分化人类诱导多能干细胞（hipsc），以创建组织贴片（如心脏、神经元等）。然后，获得的组织贴片（或其他组织培养插入物）可以集成到芯片上组装的器官中。两种不同细胞类型的共培养也是可能的为了在细胞两侧播种，需要一层基质凝胶和胶原蛋白混合比例取决于电池类型。
如果您想了解有关可用区域性修补程序的更多信息，请单击此处！
安装程序
组装完成后，微腔和微通道在芯片的两个塑料板中形成图案，以创建两个独立的微流控系统：由组织贴片分离的上腔和下腔。这两个微流控室可以用不同的培养基进行灌注。这样就可以形成一个组织界面来模拟肺泡、胃、肠、肾、肝、脑血、皮肤功能等。芯片内的组织可以通过显微镜观察或采集用于下游分析。
Organ-on-a-Chip片上器官完整设置
Description
An ideal Organ-on-a-Chip should be easy to use and with a reversible access to cells in chip for downstream analysis. Hence our Organ-on-Chip meets all these demands. Available in Small pack (1 chip - 1 clamp - 4 patches) or Large pack (4 chips - 1 clamp - 16 patches).
Three components characterize this microfluidic platform:
- Microfluidic chip
- Clamp
- Culture patch
The modular microfluidic chip consists in two plastic plates in PC (Polycarbonate), each of these has four integrated magnets for reversible assembly and an elastomeric PDMS thin film to ensure good sealing up to pressures of 8 kPa. The Luer Connectors in the upper part of the chip allow medium perfusion and dynamic flow conditions during your experiments of drug screening and/or cell differentiation.
The clamp, included in the pack, ensures good sealing with high flow pressures (up to 100 kPa). The chip can be placed in a hand screwed clamp for assembling and fast operation and, at the end of your experiment in dynamic flow conditions, the tissue patch can be easily removed by disassembling the clamps and the microfluidic chip.
The culture patch is a monolayer of nanofibers and support the organization of cells in a functional 3D microtissue. Culture patches are included in the setup and nanofibers can be made of two different natural polymers to mimic the extracellular matrix:
- gelatin
- collagen
Please, contact us if you want a custom culture patch made of synthetic biocompatible polymers:
- PLGA poly(lactic-co-glycolic acid)
- PCL polycaprolactone
- PMGI polydimethylglutarimide
Thus, different patches can be used to culture cells or to differentiate Human Induced Pluripotent Stem Cells (hiPSC) in dish or microplates in order to create your tissue patch (e.g. cardiac, neuron, etc). Then, the obtained tissue patch (or other tissue culture inserts) can be integrated in the assembled Organ-on-Chip. A co-culture of two different cell types is possible as well. In order to seed the cells on both sides a coating of matrigel and collagen is needed. The ratio of the mix will be cell type dependent.
Click here if you want to know more about the available culture patches!
Setup
Once the assembly is completed, micro-chambers and micro-channels are patterned in both plastic plates of the chip to create two independent microfluidic systems: the upper and the lower chambers separated by the tissue patch. These two microfluidic chambers can be perfused with different culture media. Thus a tissue interface can be formed to simulate alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc. Tissues inside the chip can be observed by microscopy or harvested for downstream analysis.
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