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详细介绍
微流控芯片上的器官（仅芯片）-鲁尔
描述
一个理想的芯片上的器官应该易于使用，并且可以可逆地进入芯片中的细胞进行下游分析。因此，我们的芯片器官满足所有这些要求模块化微流控芯片由两块PC（聚碳酸酯）塑料板组成，每个板上有四块可逆组装用集成磁铁和一个弹性PDMS薄膜，以确保良好的密封性。芯片上部的luer连接器允许药物筛选和/或细胞分化实验期间的介质灌注和动态流动条件。
不同的贴片可用于培养细胞或在培养皿或微孔板中分化人诱导多能干细胞（hiPSC），以创建组织贴片（如心脏、神经元等）然后，获得的组织贴片（或其他组织培养插入物）可以集成到芯片上组装的器官中。文化修补程序在这里可用！
微腔和微通道在芯片的两个塑料板上形成图案，形成两个独立的微流控系统：由组织贴片分离的上腔和下腔。这两个微流控室可以用不同的培养基进行灌注。从而形成组织界面，模拟肺泡、胃、肠、肾、肝、脑血、皮肤功能等。
芯片内的组织可以通过显微镜观察或采集用于下游分析。
微流控芯片
该芯片设计用于各种类型贴片的可逆集成。它由两块塑料板组成，每一块都有一个弹性薄膜、四个嵌入磁铁、预图案化的腔室和微通道当贴片放置在两块板之间时，四对磁铁之间的力确保在高达8千帕的压力下自动装配后，标准的luer连接板上允许一个简单的介质和药物灌注。
为确保高流量压力（高达100千帕）下的良好密封性，芯片可放置在手动螺纹夹具中进行组装和快速操作（不包括夹具，可单独订购；单击此处了解更多信息）。在动态流动条件下的实验结束时，通过拆开夹子和微流控芯片，可以很容易地取出组织补片。
更普遍地说，将4个微流控芯片与不同的组织贴片集成，可以创建一个类似于身体的循环或微生理系统，以研究不同组织（芯片上的身体）的相互影响。例如，可以将人的肠、肝、皮肤和肾细胞与一个中等循环回路联合培养，这将为药物筛选提供一个可靠的平台。
微流控芯片上的器官（仅芯片）-鲁尔
Description
An ideal Organ-on-Chip should be easy to use and with a reversible access to cells in chip for downstream analysis. Hence our Organ-on-Chip meets all these demands. The modular microfluidic chip consists in two plastic plates in PC (Polycarbonate), each having four integrated magnets for reversible assembly and an elastomeric PDMS thin film to ensure good sealing. The Luer Connectors in the upper part of the chip allow medium perfusion and dynamic flow conditions during your experiments of drug screening and/or cell differentiation.
Different patches can be used to culture cells or to differentiate Human Induced Pluripotent Stem Cells (hiPSC) in dish or microplates in order to create your tissue patch (e.g. cardiac, neuron, etc). Then, the obtained tissue patch (or other tissue culture inserts) can be integrated in the assembled Organ-on-Chip. Culture patches are available here!
Micro-chambers and micro-channels are patterned in both plastic plates of the chip to create two independent microfluidic systems: the upper and the lower chambers separated by the tissue patch. These two microfluidic chambers can be perfused with different culture media. Thus a tissue interface can be formed to simulate alveolar, stomach, intestine, kidney, liver, brain-blood, skin functions, etc.
Tissues inside the chip can be observed by microscopy or harvested for downstream analysis.
Microfluidic chip
This chip was designed for reversible integration of various types of patches. It consists in two plastic plates, each having an elastomeric thin film, four embedded magnets, and pre-patterned chambers and micro-channels. When a patch is placed between the two plates, the force between the four pairs of magnets ensures auto-assembling at pressures up to 8 kPa. Finally, the standard Luer Connectors of the upper plate allow an easy medium and drug perfusion.
To ensure good sealing with high flow pressures (up to 100 kPa), the chip can be placed in a hand screwed clamp for assembling and fast operation (the clamp is not included and can be ordered separately; click here for additional information). At the end of your experiment in dynamic flow conditions, the tissue patch can be easily removed by dissembling the clamps and the microfluidic chip.
More generally, integration of 4 microfluidic chips with different tissue patches will allow creating a body-like circulatory or micro-physiological system to investigate the mutual influences of different tissues (a Body-on-Chip). For example, a co-culture of human intestine, liver, skin and kidney cells with a medium circulation loop could be assembled and it will represent a reliable platform for drug screening.
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