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详细介绍
3D Cell Culture Chip 三维细胞培养芯片
材料
芯片采用生物相容性聚二甲基硅氧烷（PDMS）制成，具有透气性，保证了CO2和O2的交换薄玻璃载体是一种具有高透明、低自荧光等优良光学性能的显微镜载玻片。这允许通过光学显微镜、荧光显微镜和共焦显微镜以时间和空间分辨率直接观察生物样品。
技术特点
边沟中间沟
高度150微米150微米
宽度
3250微米
1125微米（狭窄部分）
3375微米（宽部分）
体积（建议注射）
20微升20微升
入口直径1000微米1000微米
芯片表面没有涂层。根据您的应用，并终改善细胞粘附性，您可以使用聚合物和/或蛋白质溶液来覆盖通道表面。
这种微流控装置还可以与细胞嵌入水凝胶（如I型胶原、IV型胶原、纤维蛋白、基质凝胶等）中，以重建可靠的生物微环境。
入口直径为1毫米，可通过普通吸管轻松注入细胞悬液20微升是每个通道/腔室的推荐体积；这将允许在实际注射和*填充时有少量额外的微升。
此外，还可以使用直接安装在入口的1/16“管子在动态流动条件下进行研究。
好处
使用方便
生理相关微环境
单细胞分辨率
直接可视化
时空分辨率
定性和定量评估
3D Cell Culture Chip 三维细胞培养芯片
Materials
The IC-Chip is made of biocompatible polydimethylsiloxane (PDMS) and is gas-permeable to ensure the CO2 and O2 exchange. The support in thin glass is a microscope slide with excellent optical properties such as high transparency and low autofluorescence. This allows a direct observation of your biological sample with temporal and spatial resolution by using optical, fluorescent and confocal microscopes.
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Technical features
| Side Channels | Middle Channel | |
| Height | 150 µm | 150 µm |
| Width | 3250 µm | 1125 µm (the narrow parts) 3375 µm (the wide parts) |
Volume (recommended for injection) | 20 µL | 20 µL |
| Diameter of inlets | 1000 µm | 1000 µm |
The IC-Chip has not coated surfaces. According to your application and to eventually improve cell adhesion, you can coat the channel surface by using your solutions of polymers and/or proteins.
This microfluidic device can be used also with cells embedded in hydrogels such as Collagen I, Collagen IV, Fibrin, Matrigel, etc. to recreate a reliable biological microenvironment.
The inlet diameter of 1 mm allows an easy injection of the cell suspension through common pipettes. 20 µL is the recommended volume for each channel/chamber; this will allow to have few extra µL for a practical injection and complete filling.
Additionally, it would be possible to perform studies in dynamic flow conditions by using 1/16” tubing directly fitted in the inlets.
Benefits
- Easy-to-use
- Physiologically relevant microenvironment
- Single cell resolution
- Direct visualization
- Temporal and spatial resolution
- Qualitative and quantitative assessments
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