8-Well Cell Settling Chamber

8-Well Cell Settling Chamber该腔室由两个精密加工的丙烯酸板，一个硅酮垫圈，和组装硬件。较低的平板有凹槽，可容纳25 x 75毫米的玻璃显微镜载玻片。上片有8个通孔，上片夹在下片显微镜载玻片上形成280l孔。垫圈和一张预冲滤纸(股票号DC8WF1)夹在两个板之间，导致细胞悬浮液的液体吸走，因为细胞落在幻灯片上，留下细胞的位置干燥。为了获得好的结果，可以在8个沉降点(编号DC8SC)周围使用增强结合性能的显微镜载玻片和疏水性掩膜。这些幻灯片改善了细胞粘附性，也限制了管道解决方案的个别地点。然后可以在没有串扰的情况下对固定的单元应用不同的解决方案。在处理淋巴细胞等密度较低的细胞时，应在装有微板载体的离心机中旋转腔体。离心机附件包(股票编号DC8AP)提供了一个适配器和配重用于此目的。每个腔室都附带一个DC8协议供其使用和护理。滑槽尺寸:DC8 32mm2 280 L 25 x 75 x 1 mm

8-Well Cell Settling Chamber

The chamber consists of two precision-machined acrylic plates, a silicone gasket, and assembly hardware. The lower plate has a recess to fit a 25 x 75mm glass microscope slide. The upper plate has eight through holes, which form 280µL wells when the upper plate is clamped onto the microscope slide in the lower plate. The gasket and a sheet of prepunched filter paper (Stock # DC8WF1) pinched between the plates cause liquid in the cell suspension to wick away as the cells settle onto the slide, leaving the cell sites dry.

For best results microscope slides with enhanced binding properties and a hydrophobic mask around the eight settling sites (Stock # DC8SC) are available. These slides improve cell adherence and also restrict pipetted solutions to individual sites. Different solutions can then be applied to settled cells without crosstalk.

When working with cells of relatively low density, such as lymphocytes, the chamber should be spun in a centrifuge equipped with a microplate carrier. A centrifuge accessory pack (Stock # DC8AP) provides an adapter and counterweight for this purpose.

Accompanying each chamber is a DC8 protocol for its use and care.