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详细介绍
一种模拟细胞生长过程中的机械应力装置:美国B-Bridge细胞牵拉仪（STREX）。
共有型号：
ST-140-04
ST-140-10
ST-150
ST-160
ST-190-XY
ST-195
B-Bridge细胞牵拉仪可精准控制施加在细胞上的应力级别，使张力腔室中的种子细胞被拉伸或压缩，更好的模拟了自然动态生理环境。ST-190-XY有168个自定义应变参数（8个拉伸/压缩比 8个频率），其余皆为64个自定义应变参数（8个拉伸/压缩比 8个频率）。
型号：
ST-150
ST-160
ST-190-XY
ST-195
可与显微镜联用
型号：
ST-140-04
ST-140-10
ST-150
ST-160
ST-195
可单向控制施加在细胞上的应力
型号：
ST-190-XY
可沿X轴和Y轴压缩双向控制施加在细胞上的应力
型号ST-140-04采用4cm2硅胶腔室
型号ST-140-10采用10cm2硅胶腔室
型号ST-150采用4cm2硅胶腔室
型号ST-160采用0.2cm2硅胶腔室
型号ST-190-XY采用10cm2硅胶腔室
型号ST-195采用4cm2硅胶腔室
ST-150
ST-160
ST-190-XY
ST-195
Stretch Chambers
The exceptional physical and chemical properties of the silicone elastomer PDMS (polydimethylsiloxane) create a specially flexible thin-membrane chamber.
Stretch chambers are flexible culture dishes developed for inducing mechanical stimulation on cultured cells.
- High reproducibility: Spring-like PDMS chambers bounce back from stretching and compressing. With their original properties intact. The chambers show good reproducibility in applications which need continuous mechanical stretching over prolonged periods of time.
- Superior transparency: An optically transparent, ultra-thin (100-200 μm) membrane at the well bottom. It not only makes stretch chambers compatible with optical microscopy techniques, but with fluorescence detection and microscopy as well.
Uniformity of direction and force are issues of crucial concern in cell stretching.
Stretching systems and chambers with insufficient properties can cause extraneous stretching on the wrong axis and generate a secondary load in that direction.
Also, these systems may not apply equal stress to all the cells in the chamber. This makes it difficult to accuray gauge the effect of the stretch stimulus across the unevenly treated sample.
The STREX Cell Stretching System is designed to achieve stretching in a single, parallel direction, with only a weak secondary load.
Research has demonstrated that the STREX system enables highly reproducible cyclic stretching over prolonged periods at ratios of 1-20%. (Ref.)
Because the methyl groups align themselves to the surface, the stretch chamber is highly water-repellent, meaning that cells will not adhere without pre-treatment of the chamber.
Thus, the PDMS chamber surface needs to be coated with extracellular matrix to successfully adhere the culture cells. Fibronectin, collagen, gelatin and laminin, among others, can be used for this purpose.
STREX offers standard stretch chambers in two sizes, the 4 cm² and 10cm² models. Specialized versions are also available, including multi-well, gel-stretching, and transwell experiment chambers.
Ref. Naruse. K., Yamada T., Sai X.R., Hamaguchi M., Sokabe M. (1998), Pp125FAK is required for stretch dependent morphological response of endothelial cells. Oncogene,17:455-463.
Stretch Chambers
4cc Chamber
STB-CH-04
Corresponding models:
STB-140-04
STB-150
STB-10-04
Culture area:
2.0 x 2.0 x 1.0 cm
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Real Time Imaging
STB-CH-0.02
Corresponding models:
STB-150W
up to 40x magnification
(In order to see at this magnification the motor must be turned off briefly.)
Culture area:
1.0 x 1.0 x 0.02 cm
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Biaxial Stretch Chamber
STB-CH-04-XY
Corresponding models: STB-190-XY
Culture area:
2.0 x 2.0 x 1.0 cm
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10cc Chamber
STB-CH-10
Corresponding models:
STB-140-10
STB-10-10
Culture area:
3.2 x 3.2 x 1.0 cm
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Three Dimensional Culture
STB-CH4-G
Corresponding models:
STB-140-04
STB-10-04
Culture area:
1.0 x 1.0 x 0.02 cm
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Tissue Samples
STB-CH-0.06
Corresponding models:
STB-140-04
STB-140-10
For Pulmonary Tissue
Culture area:
1.0 x 0.6 x 1.0 cm
STB-CH-4W
Corresponding models:
STB-140-10
STB-10-10
Culture area:
1.5 x 1.5 x 1.0 cm
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STB-CH4-GP
Corresponding models: STB-140-04 STB-10-04
4 microchambers 20 x 20mm
Culture volume: 4cc
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STB-CH-1.5
Corresponding models:
STB-140-04
STB-140-10
For Achilles Tendon
Culture area:
1.5 x 1.0 x 1.5 cm
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Custom chambers available, contact us for details.
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