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产品文献:猪IL-6,IL-1β,TNF-α ELISA试剂盒引用文献
阅读：98 发布时间：2024-4-26| 提 供 商 | 上海恒远生物科技有限公司 | 资料大小 | 14.9MB |
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【文献标题】Manipulation of hydrophobic motifs and optimization of sequence patterns to design high stability peptides against piglet bacterial infections
【作者】Peng Tan , Zhihong Sun , Qi Tang , et.al
【作者单位】中国农业大学（China Agricultural University）
【文献中引用产品】
猪白细胞介素6（IL-6）ELISA试剂盒
猪白细胞介素1β(IL-1β)ELISA试剂盒
猪肿瘤坏死因子α（TNF-α）ELISA试剂盒
【关键词】Antibacterial biomaterials ,High stability ,Manipulation of hydrophobic motifs ,Optimization of sequence patterns ,In vivo efficacy
【DOI】doi.org/10.1016/j.nantod.2023.101793
【影响因子(IF)】18.962
【出版期刊】《Nano Today》
【产品原文引用】
Healthy female weaned piglets (Duroc × Landrace × Large white, weighing ≈ 8 kg) were randomly divided into four groups (5 per group). One group of healthy piglets received no treatment and served as a control. Piglets in the other three groups were intraperitoneally injected with 15 mL of E. coli ATCC25922 with an OD600 = 0.15, and 2 h later, they were given an intraperitoneal injection of saline, peptide HLFP-5 (2.5 mg/kg), or HLWP-4 (2.5 mg/kg), respectively, with an injection volume of 10 mL. After 12 h, all piglets were euthanized. The blood of all piglets was collected and the levels of inflammatory factors in serum were determined by the ELISA method. The inflammatory factor kit was purchased from Shanghai Hengyuan Biological Technology Co., Ltd. Partial livers, kidneys, spleens, and lungs of bacteria-infected piglets were collected for bacterial enumeration studies by standardized localization. Furthermore, some organ samples of piglets were stained with H&E for histological examination.
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