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上海源叶生物科技有限公司
主营产品： S30260异硫氰酸胍,30259盐酸胍,嗜热菌蛋白酶 |
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15921386130
公司信息
- 联系人：
- 何小姐
- 电话：
- 86-021-61559134
- 手机：
- 15921386130
- 传真：
- 86-021-55068248
- 地址：
- 上海市松江区长塔路465号6幢
- 邮编：
- 200433
- 网址：
- www.shyuanye.com
S26102
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更新时间：2024-07-02 07:01:40浏览次数：161
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- 产品描述：
PS 48 has been shown to be a PKB Kinase (PDK1) activator (Kd: 10.3 μM). This compound selectively binds to the PIF-binding pocket of PKB Kinase (PDK1).
- 细胞实验： Human renal mesangial cells (HRMCs) are cultured with 1640 media, containing 10% fetal bovin serum at 37 °C in 5% CO2. Cells are cultured with D-glucose at normal (5.5 mM) or high (25 mM) concentrations in serum-free medium. D-Mannitol (25 mM) is used for a control of osmolality. TP is reconstituted in 0.01% DMSO and freshly diluted with culture medium to 10 ug/L before using. To determine the specific role of PDK1 in TP-potentiated anti-proliferation, 5 μM PS48 (MedChem Express, USA) is applied following the treatment of TP. MTT assay is used to detect cell proliferation. HRMCs are seeded at a density of 1x105/mL into 96-well plates. After 12, 24, 48 and 72 h incubation with different compounds, 20 uL MTT (5 mg/mL) is added to each well. Cells are then cultured for an additional 2 h and subsequently lysed using DMSO (150 uL/well). When the formazan crystals completely dissolve, the optical density (OD) is measured at 570 nm. The arithmetic mean OD of six wells for each group is calculated.
- 参考文献：
1. Hindie V, et al. Structure and allosteric effects of low-molecular-weight activators on the protein kinase PDK1. Nat Chem Biol. 2009 Oct;5(10):758-64.
2. Han F, et al. Triptolide Suppresses Glomerular Mesangial Cell Proliferation in Diabetic Nephropathy Is Associated with Inhibition of PDK1/Akt/mTOR Pathway. Int J Biol Sci. 2017 Sep 21;13(10):1266-1275.
- 保存条件： -20℃
- 配置溶液浓度参考：
1mg 5mg 10mg 1 mM 3.487 ml 17.437 ml 34.873 ml 5 mM 0.697 ml 3.487 ml 6.975 ml 10 mM 0.349 ml 1.744 ml 3.487 ml 50 mM 0.07 ml 0.349 ml 0.697 ml
- 注意：部分产品我司仅能提供部分信息，我司不保证所提供信息的性，仅供客户参考交流研究之用。
