CF488A是一种针对488nm氩离子激光器优化的绿色荧光染料。在通常的检测条件下，CF488A的亮度至少和Alexa Fluor 488 相当。但CF488A相对于Alexa Fluor 488有一个明显的优势，用CF488A制备的荧光偶联抗体的生物学特异性更好。Alexa Fluor 488本身带有多个负电荷，将会显著的改变被标记蛋白的等电点，因此也就会改变用它标记的荧光偶联抗体的生物学特异性。与此相反，CF488A几乎不带电荷，用它制备的荧光偶联抗体可以保证检测的特异性并提高信噪比。CF488A的另一个优点是它的峰值发射波长比Alexa Fluor 488短10nm，比传统的FITC(FAM)染料短15nm。因此，在多波长检测的时候，CF488A可以显著减少在红色通道的溢出效应，从而减小对红色荧光的干扰。
表A-C 为CF488A产品目录，我们提供全套的CF488A荧光偶联二抗，抗体标记试剂盒，以及其他生物分子偶联CF488A的产品，包括：鬼笔环肽，膜联蛋白 V和a-银环蛇毒素等偶联产品。
南京赛泓瑞生物为Biotium公司 的一级代理商，： :
技术资料：
优势：
| 图1.CF488A（蓝色）， Alexa Fluor 488（绿色）和FITC（红色）偶联羊抗鼠IgG 二抗在PBS溶液中的激发和发射光谱。 |
图2. 用胞外CD3抗体或同型对照抗体（BD Biosciences）分别标记Jurkat细胞，然后分别用偶联图中所示厂商荧光基团的羊抗鼠IgG二抗染色。插图显示的是用Beckman Coulter FC-500检测到的细胞计数的中位数荧光值。直方图显示的是非别用CF488A（绿色），AlexaFluor488（蓝色），DyLight488 Pierce（紫色）和DyLight488 KPL（红色）染色的CD3标记细胞，使用BD FACS Calibur 在FL1通道上检测。 | 图3. 用fibronectin抗体标记人的心肌冰冻切片，并分别使用CF488A，Cy 2 和Alexa Fluor 488偶联的羊抗兔IgG作为二抗染色。感谢来自德国Bad Nauheim (Hessen) Max-Planck-Institute für Herz- und Lungenforschung (W.G. Kerckhoff-Institut)的Sawa Kostin博士提供了上述图片。 |
Table A. CF488A Dye-labeled Secondary Antibody Conjugates
| Cat# | Product Name | Unit Size | Unit Price | 品 牌 |
20289 | Bovine serum albumin, CF488A conjugate | 5x1mg | ￥1999 | 美国Biotium |
20293 | CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20293-1 | CF488A Bovine anti-goat IgG(H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美国Biotium |
20225 | CF488A Chicken Anti-Goat IgG (H+L), 2mg/mL | 0.5 mL | ￥1764 | 美国Biotium |
20225-1 | CF488A Chicken Anti-Goat IgG (H+L), 2mg/mL | 50 uL | ￥764 | 美国Biotium |
20208 | CF488A Chicken Anti-Mouse IgG (H+L), 2mg/mL | 0.5 mL | ￥1764 | 美国Biotium |
20208-1 | CF488A Chicken Anti-Mouse IgG (H+L), 2mg/mL | 50 uL | ￥706 | 美国Biotium |
20209 | CF488A Chicken Anti-Rabbit IgG (H+L), 2mg/mL | 0.5 mL | ￥1764 | 美国Biotium |
20209-1 | CF488A Chicken Anti-Rabbit IgG (H+L), 2mg/mL | 50 uL | ￥706 | 美国Biotium |
20166 | CF488A Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20166-1 | CF488A Donkey Anti-Chicken IgY (IgG) (H+L), highly crossed-adsorbed, 2mg/mL | 50 uL | ￥882 | 美国Biotium |
20016 | CF488A Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20016-1 | CF488A Donkey Anti-Goat IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL | ￥882 | 美国Biotium |
20169 | CF488A Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20169-1 | CF488A Donkey Anti-Guinea Pig IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美国Biotium |
20074 | CF488A Donkey Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20074-1 | CF488A Donkey Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美国Biotium |
20014 | CF488A Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20014-1 | CF488A Donkey Anti-Mouse IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL | ￥882 | 美国Biotium |
20015 | CF488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20015-1 | CF488A Donkey Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL | ￥882 | 美国Biotium |
20027 | CF488A Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20027-1 | CF488A Donkey Anti-Rat IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL | ￥882 | 美国Biotium |
20024 | CF488A Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20024-1 | CF488A Donkey Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL | ￥882 | 美国Biotium |
20020 | CF488A Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20020-1 | CF488A Goat Anti-Chicken IgY (IgG) (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美国Biotium |
20017 | CF488A Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL | 0.5 mL | ￥1764 | 美国Biotium |
20017-1 | CF488A Goat Anti-Guinea Pig IgG (H+L), 2 mg/mL | 50 uL | ￥706 | 美国Biotium |
20022 | CF488A Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20022-1 | CF488A Goat Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美国Biotium |
20010 | CF488A Goat anti-mouse IgG (H+L), 2 mg/mL | 0.5 mL | ￥1764 | 美国Biotium |
20010-1 | CF488A Goat anti-mouse IgG (H+L), 2 mg/mL | 50 uL | ￥706 | 美国Biotium |
20011 | CF488A Goat anti-mouse IgG (H+L), F(ab')2 fragment, 2 mg/mL | 0.25 mL | ￥1411 | 美国Biotium |
20302 | CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL | 0.5 mL | ￥2764 | 美国Biotium |
20302-1 | CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed (min X Rat), 2 mg/mL | 50 uL | ￥1176 | 美国Biotium |
20018 | CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20018-1 | CF488A Goat anti-mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美国Biotium |
20246 | CF488A Goat anti-mouse IgG1, (?31), 2 mg/mL | 0.25mL | ￥2293 | 美国Biotium |
20256 | CF488A Goat anti-mouse IgG2a, (?32a), 2mg/mL | 0.25mL | ￥2293 | 美国Biotium |
20256-1 | CF488A Goat anti-mouse IgG2a, (?32a), 2mg/mL | 50 uL | ￥1000 | 美国Biotium |
20266 | CF488A Goat anti-mouse IgG2b, (?32b), 2mg/mL | 0.25mL | ￥2293 | 美国Biotium |
20012 | CF488A Goat Anti-Rabbit IgG (H+L), 2 mg/mL | 0.5 mL | ￥1764 | 美国Biotium |
20012-1 | CF488A Goat Anti-Rabbit IgG (H+L), 2 mg/mL | 50 uL | ￥706 | 美国Biotium |
20013 | CF488A Goat Anti-Rabbit IgG (H+L), F(ab')2 fragment, 2 mg/mL | 0.25 mL | ￥1411 | 美国Biotium |
20019 | CF488A Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20019-1 | CF488A Goat Anti-Rabbit IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美国Biotium |
20023 | CF488A Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20023-1 | CF488A Goat Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美国Biotium |
20028 | CF488A Goat Anti-Swine IgG (H+L), 2 mg/mL | 0.5 mL | ￥1764 | 美国Biotium |
20028-1 | CF488A Goat Anti-Swine IgG (H+L), 2 mg/mL | 50 uL | ￥706 | 美国Biotium |
20228 | CF488A Monoclonal Mouse Anti-6X His IgG, 1mg/mL | 50 uL | ￥3352 | 美国Biotium |
20204 | CF488A Monoclonal Mouse Anti-Biotin, 2 mg/mL | 0.25 mL | ￥2117 | 美国Biotium |
20204-1 | CF488A Monoclonal Mouse Anti-Biotin, 2 mg/mL | 50 uL | ￥1000 | 美国Biotium |
20210 | CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL | 0.25 mL | ￥2293 | 美国Biotium |
20210-1 | CF488A monoclonal mouse anti-fluorescein (FITC) IgG, 2 mg/mL | 50 uL | ￥1117 | 美国Biotium |
20215 | CF488A monoclonal mouse anti-GFP IgG, 1 mg/mL | 0.1 mL | ￥2587 | 美国Biotium |
20079 | CF488A Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/mL | 0.5 mL | ￥1764 | 美国Biotium |
20079-1 | CF488A Rabbit Anti-Chicken IgY (IgG) (H+L), 2mg/mL | 50 uL | ￥706 | 美国Biotium |
20021 | CF488A Rabbit Anti-Goat IgG (H+L), 2 mg/mL | 0.5 mL | ￥1764 | 美国Biotium |
20021-1 | CF488A Rabbit Anti-Goat IgG (H+L), 2 mg/mL | 50 uL | ￥706 | 美国Biotium |
20238 | CF488A Rabbit anti-HA (Hemagglutinin) IgG, 1 mg/mL | 50 uL | ￥3352 | 美国Biotium |
20071 | CF488A Rabbit Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20071-1 | CF488A Rabbit Anti-Human IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美国Biotium |
20026 | CF488A Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20026-1 | CF488A Rabbit Anti-Mouse IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美国Biotium |
20025 | CF488A Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20025-1 | CF488A Rabbit Anti-Rat IgG (H+L), highly cross-adsorbed, 2 mg/mL | 50 uL | ￥882 | 美国Biotium |
20172 | CF488A Rabbit Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL | 0.5 mL | ￥2117 | 美国Biotium |
20172-1 | CF488A Rabbit Anti-Sheep IgG (H+L), highly cross-adsorbed, 2mg/mL | 50 uL | ￥882 | 美国Biotium |
Table B. Other CF488A dye-labeled Products
| Cat# | Product Name | Unit Size | Unit Price | 品 牌 |
29005 | Annexin V, CF488A conjugate, 50 ug/mL | 0.5 mL | ￥2587 | 美国Biotium |
00005 | CF488A a-Bungarotoxin | 0.5 mg | ￥3528 | 美国Biotium |
29016 | CF488A Concanavalin A (Con A) | 5x1 mg | ￥1529 | 美国Biotium |
29034 | CF488A Streptavidin | 1 mg | ￥1882 | 美国Biotium |
00042 | Phalloidin, CF488A conjugate | 300 U | ￥3587 | 美国Biotium |
29022 | Wheat Germ Agglutinin, CF488A conjugate | 5x1mg | ￥2940 | 美国Biotium |
29022-1 | Wheat Germ Agglutinin, CF488A conjugate | 1mg | ￥706 | 美国Biotium |
Table C. Reactive CF488A Dyes and Labeling Kits
| Cat# | Product Name | Unit Size | Unit Price | 品 牌 |
92152 | CF488A hydrazide | 1 mg | ￥1764 | 美国Biotium |
92022 | CF488A maleimide | 1 umole | ￥2411 | 美国Biotium |
92213 | CF488A SE protein labeling kit | 3 labelings | ￥4292 | 美国Biotium |
92120 | CF488A succinimidyl ester | 1 umole | ￥2646 | 美国Biotium |
92051 | CF488A, aminooxy | 1 mg | ￥4586 | 美国Biotium |
92253 | Mix-n-Stain CF488A antibody labeling kit, 1x(20-50 ug) labeling | 1 labeling | ￥1235 | 美国Biotium |
92233 | Mix-n-Stain CF488A antibody labeling kit, 1x(50-100 ug) labeling | 1 labeling | ￥1352 | 美国Biotium |
92273 | Mix-n-Stain CF488A antibody labeling kit, 1X(5-20ug) labeling | 1 labeling | ￥1117 | 美国Biotium |
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