Ponceau S
CAS Number: 6226-79-5
Storage Temperature: Room Temperature
Product Description :
Appearance: Deep red crystalline powder
Molecular formula: C 22 H 12 N 4 Na 4 O 13 S 4
Molecular weight: 760.6
Ponceau S is a red, water-soluble, anionic diazo dye that is made by coupling diazotized
4-ami,1’-azobenzene-3,4’-disulfonic acid (Acid Yellow 9) with 2-naphthol-3,6-disulfonic acid(R acid)
and converting the coupled product to the tetrasodium salt.
Ponceau S is a negative stain, which binds to the positively charged amino groups of the protein and is
widely used in gel electrophoresis. It also binds non-covalently to non-polar regions in the protein.
Ponceau S may not be as sensitive of a protein stain as Coomassie Blue. It is superior to Acid Fuchsin in
the VanGieson stain.
Preparation Instructions :
It is soluble in water (1 mg/ml), yielding a clear solution.
Procedure:
A solution as low as 1% ponceau S can be made in 7% acetic acid (w/w) for staining polyacrylamide gels.
The gel can be left in this solution for eight hours. However, prior to placing the gel in this staining mixture,
the proteins on the gels should be fixed with a fixative agent such as methanol:acetic acid or TCA.
Destaining of the gel can occur in 7% (v/v) acetic acid:water mixture with many changes of the
destaining solution.
Storage/Stability:
Recommended storage is at room temperature. The shelf-life should be at least two years.
Precautions and Disclaimer :
For Laboratory Use Only. Not for drug, household or other uses.

Ponceau S
丽春红S
H i gh Puri t y G rade
产品编号 规 格 价 格
P8330-5 5g 60.00
P8330-10 10g  100.00
说明：生物染色，蛋白质电泳后染色。丽春红可用于检测联结于
NC膜及PVDF膜上的蛋白质，不能用于Nylon膜。
别名：猩红S；Fast ponceau 2B；Ponceau Sextra
分子式：C 22 H 12 N 4 Na 4 O 13 S 4 分子量：760.61
C AS＃：6226-79-5
外观：棕色粉末
特性：水分(%)：≤6.0
溶解性 ：溶于水，配制方法有两种，一种是0.1％丽春红
S(w/v)，5％冰醋酸；另一种是2％丽春红S(w/v),3%
TCA,3%Sulfosalicylic acid。
R：36/37/38 S：36/37/39
储存条件：室温