重组人巨噬细胞集落刺激因子（rHuM-CSF）_以色列Prospec细胞因子-上海易佰聚经贸有限公司

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CYT-308

重组人巨噬细胞集落刺激因子（rHuM-CSF）

2μg/10μg/1mg

Prospec

Background:
	Macrophage colony stimulating factor （M-CSF） is one of the glycoproteins called colony-stimulating factors （CSFs）.A mononuclear phagocyte colony-stimulating factor （M-CSF） synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo
	Description :
	Recombinant Human M-CSF produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KD Recombinant M-CSF is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	The lyophilized protein （1mg/ml） was lyophilized with 0.1gr HSA and 0.6gr Manntiol.
	Solubility:
	It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution M-CSF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Glu-Val-Ser.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec’s M-CSF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 5ng/ml, corresponding to a Specific Activity of 2 x 10⁵ IU/mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of M-CSF.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280. 2. Analysis by RP-HPLC, using a standard solution of M-CSF as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.