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重组人可溶性CD4 (rsCD4)

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更新时间:2025-05-02 13:04:00浏览次数:901次

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CYT-304重组人可溶性CD4 (rsCD4) 2μg/10μg/1mg Prospec

 

 

Background:
 CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MHC (Major Histocompatibility Complex) Class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as HIV receptors, binding directly to the envelope protein gp120 on HIV. Thymocytes which are CD4 (+)and CD8 ( - )produce factors such as IL-4 , IL-5 ,IL-10 and IFN-gamma

 
Description :
 Recombinant Human sCD4 produced in Baculovirus is a single, glycosylated polypeptide chain containing 365 amino acids and having a non glycosilated molecular mass of 40,000 Dalton. As a result of glycosilation the sCD4 migrates as a 46,000 dalton protein on standard SDS-PAGE. Detected By Western Imunno Blots anti cd4 monoclonal antibody.
Recombinant soluble CD4 is purified by proprietary chromatographic techniques.
 
Physical Appearance:
 Sterile Filtered colorless liquid formulation.
 
Formulation:
 Recombinant Human Soluble CD-4 solution contains 10mM sodium phosphate buffer pH-7.2, 300mM sodium chloride and 0.01% tween.
Stability:
 rHusCD4 although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
 
Purity:
 Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
 
Biological Activity:
 ProSpec’s Recombinant Human Soluble CD-4 is fully biologically active when compared to standard. Immune reactive in ELISA using monoclonal anti CD4 in gpl2O capture ELISA assays.
 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of soluble CD4.
 
Protein content:
 Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of CD4 as a Reference Standard.
 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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