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Pierce Co-IP Kit介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

Pierce Co-IP Kit现货供应

Pierce Co-IP Kit品牌介绍

美国Pierce公司（www.pierce.com）是蛋白质化学、免疫学和层析等很多方面的和*。http://www.thermo.com.cn/Category828.html

主要产品包括：

1）化学发光体和比色底物

2）分析试剂

3）荧光标记探针

4）酶联免疫分析试剂盒等

Pierce Co-IP Kit仅用于科研

The Thermo Scientific Pierce Co-Immunoprecipitation （Co-IP） Kit enables isolation of native protein complexes from a lysate or other complex mixture by directly immobilizing purified antibodies onto an agarose support.

Co-IP is a common approach to study protein:protein interactions that uses an antibody to immunoprecipitate the antigen （bait protein） and co-immunoprecipitate any interacting proteins （prey proteins）. Traditional co-IP methods that use Protein A or G result in co-elution of the antibody heavy and light chains that may co-migrate with relevant bands, masking important results. The Pierce Co-IP Kit resolves this issue by covalently coupling antibodies onto an amine-reactive resin. The kit includes optimized buffers for protein binding and recovery, reagents to perform control experiments and efficient spin columns and collection tubes, which shorten the protocol and minimize handling and mixing.

Highlights:

• Works for any purified antibody – perform co-IP experiments using any purified antibody regardless of species or Ig class （chicken IgY, human IgE, mouse IgG1, IgM, etc.）; no dependence on Protein A and Protein G affinity binding
• No antibody interference – covalent attachment method and non-reducing elution system yields pure co-IP products without contamination by the IP antibody
• Reusable antibody beads – covalent antibody immobilization allows prepared IP resin to be reused and provides potential savings of costly antibody
• Complete Kit – all reagents for antibody immobilization and many IP experiments, including binding and wash buffers, elution buffer and convenient microcentrifuge spin columns to make resin manipulations easier and more efficient.
• Control beads included – underivatized agarose beads provided for use as negative control for nonspecific binding
• Versatile – co-IP method is compatible with any physiolgical （non-denaturing） protein sample buffer that is compatible with the specific antibody-antigen and protein interaction complex.