PIERCE,Pierce（R） Classic IP Kit 返回列表页

The Thermo Scientific Pierce Classic IP Kit provides all the necessary reagents, spin cups and collection tubes to perform successful immunoprecipitation （IP） experiments with ease.

The kit uses high-capacity Protein A/G agarose affinity resin for efficient binding of most species and subclasses of IP antibodies. The included IgG elution buffer provides milder and less denaturing recovery of antibody:antigen complexes than the traditional method of boiling in reducing sample buffer for SDS-PAGE, facilitating a greater variety of methods for subsequent analysis. The microcentrifuge spin column format helps to ensure effective washing and separation of samples from the beaded agarose affinity resin.

Highlights:

• Assay consistency – Pierce Spin Columns （see dimensions） eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes
• Fast – Immunoprecipitate （IP） in less than one hour
• Mild elution conditions – Recover antigen without harsh detergents or reducing agents
• Easy-to-follow instructions – IP target protein from crude lysate in four simple steps
• Suitable for most common antibody types – Protein A/G provides excellent binding for most mouse, rabbit, human and goat IgG subclasses
• Complete kit – includes lysis/wash buffer, binding and elution buffers, control agarose resin, sample loading buffer and spin columns