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1-ETHYL-3-(3-DIMETHYLAMINOPROPYL)CARBODIIMIDE.HCL (EDC)介绍
Thermo Scientific BCA蛋白定量产品可以用于研究蛋白:蛋白间相互作用,衡量亲和层析后的组分含量,估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。
1-ETHYL-3-(3-DIMETHYLAMINOPROPYL)CARBODIIMIDE.HCL (EDC)现货供应
PIERCE | 22981 | 1-ETHYL-3-(3-DIMETHYLAMINOPROPYL)CARBODIIMIDE.HCL (EDC) | 25 GM |
PIERCE | 23011 | (MMTS) Methyl methanethiolsulfonate, 200 mg. | 200 MG |
PIERCE | 23030 | N-ETHYLMALEIMIDE | 25 GM |
1-ETHYL-3-(3-DIMETHYLAMINOPROPYL)CARBODIIMIDE.HCL (EDC)品牌介绍
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1-ETHYL-3-(3-DIMETHYLAMINOPROPYL)CARBODIIMIDE.HCL (EDC)仅用于科研
Thermo Scientific Pierce EDC is a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation.
1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC or EDAC) is a zero-length crosslinking agent used to couple carboxyl groups to primary amines. This crosslinker has been used in diverse applications such as forming amide bonds in peptide synthesis, attaching haptens to carrier proteins to form immunogens, labeling nucleic acids through 5’ phosphate groups and creating amine-reactive NHS-esters of biomolecules. EDC reacts with a carboxyl to form an amine-reactive O-acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N-hydroxysulfosuccinimide (Sulfo-NHS), EDC can be used to convert carboxyl groups to amine-reactive Sulfo-NHS esters. This is accomplished by mixing the EDC with a carboxyl containing molecule and adding Sulfo-NHS.
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