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DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL（DTBP）介绍

Thermo Scientific BCA蛋白定量产品可以用于研究蛋白：蛋白间相互作用，衡量亲和层析后的组分含量，估算从细胞裂解液里提取的膜蛋白含量和高通量筛选融合蛋白。

DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL（DTBP）现货供应

DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL（DTBP）品牌介绍

美国Pierce公司（www.pierce.com）是蛋白质化学、免疫学和层析等很多方面的和*。http://www.thermo.com.cn/Category828.html

主要产品包括：

1）化学发光体和比色底物

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3）荧光标记探针

4）酶联免疫分析试剂盒等

DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL（DTBP）

仅用于科研

Thermo Scientific Pierce DTBP is dimethyl 3,3'-dithiobispropionimidate, a crosslinker that contains amine-reactive imidoester groups around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

Dimethyl dithiobispropionimidate （DTBP） is a homobifunctional, cleavable and membrane permeable cross-linker. It contains an amine-reactive imidoester at each end of an 8-atom spacer arm. Imidoesters react with primary amines at pH 8-10 to form stable amidine bonds. The amidine is protonated and therefore, like the lysine it modified, has a positive charge at physiological pH. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for imidoester crosslinking reagents.

DTBP crosslinks can be easily reversed by the addition of 100-150 mM DTT at 37°C for 30 minutes. DMS, the non-cleavable analog of DTBP is available for applications that require a stable, non-cleavable bond. DTSSP, the NHS-ester analog of DTBP is also available for applications that require crosslinking at physiologic pH or greater stability of the crosslinked product at elevated pH. DTBP and DMS have essentially identical crosslinking activity toward primary amines.

DTBP Highlights

• Amine-reactive imidoester reacts rapidly with any primary amine-containing molecule to form amidine bonds
• DTBP crosslinker is water soluble for compatibility with aqueous solutions
• DTBP crosslinking can be easily reversed with DTT, TCEP or other reducing agents
• High purity DTBP can be used to create high-purity conjugates

• High purity DTBP can be used to create high-purity conjugates