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北京西美杰科技有限公司
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A3778,0010 10 mg A3778,0100 100 mg A3778,0500 500 DNase I(脱氧核糖核酸酶I)
参考价 | 面议 |
具体成交价以合同协议为准
- 型号 A3778,0010 10 mg A3778,0100 100 mg A3778,0500 500
- 品牌
- 厂商性质 代理商
- 所在地 上海市
更新时间:2014-07-05 11:03:46浏览次数:867
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Synonym | Deoxyribonuclease I, [E.C. 3.1.21.1] | |||
---|---|---|---|---|
delivery form | salt-free, freeze-dried powder, chromatographically prepared | |||
origin | from bovine pancreas | |||
M | ~31000 g/mol | |||
CAS-No. | 9003-98-9 | |||
HS-No. | 35079090 | |||
EC-No. | 232-667-0 | |||
Storage | -20°C | |||
LGK | 10 - 13 | |||
Specification | ||||
Activity (Kunitz) | min. 3000 U/mg |
(2) Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) 2001. Currrent Protocols in Molecular Biology. John Wiley & Sons, N.Y.
(3) McDonald, M.R. (1955) Methods Enzymol. 2, 437-447
Deoxyribonucleases
(4) Campbell, V.W. & Jackson, D.A. (1980) J. Biol. Chem. 255, 3726-3735
The effect of bivalent cations on the mechanism of DNase I.
(5) Meinkoth, J. & Wahl, G.M. (1987) Methods Enzymol. 152, 91-94
Nick-Translation.
(6) Cobianchi, F. & Wilson, S.H. (1987) Methods Enzymol. 152, 94-110
Enzymes for modifying and labeling of DNA and RNA.
The enzyme is used in molecular biology techniques like digestion of DNA, in the RNA purification (ref. 2 Suppl. 1 pp. 4.1.4) or generating "random nicks" for "nick translation" (ref. 2 Suppl. 9 pp. 3.5.4-6) or 'footprint'-assays (ref. 2 Suppl. 7 chapter 12.4) or investigations on chromatin (ref. 2 Suppl. 48 chapter 21.4.1).
Unit definition: One unit is defined as that amount of enzyme which causes an increase of absorbance at 260 nm of 0.001 per minute at 25°C based on the method of Kunitz. DNase I is readily soluble in e. g. 0.15 M sodium chloride or in reaction buffer (e. g. 50 mM Tris · Cl, pH 7.5; 10 mM MgCl2 (single-strand 'nicks') and 10 mM MnCl2 (double-strand breakes), respecitvely; 50 μg/ml BSA; ref. 2 Suppl. 8 page 3.12.5). For storage dissolve DNase I in 50 % glycerol (w/v); 20 mM Tris · Cl, pH 7.5; 1 mM MgCl2. For stability reasons the concentrations should be at least 1 mg/ml (The maximum solubility is 10 %). This solution is stable for more than one year (ref. 2 Suppl. 8 page 3.12.5). The lyophilized form is stable for 2 - 5 years if kept at +4°C. If a solution is protease-free, DNase I will not loose significant activity at pH 5 - 7 and 62°C for 5 hours. The enzyme may be heat-inactivated (10 minutes at 99°C).
RNase-free DNase I: Dissolve DNase I at 1 mg/ml in 0.1 M iodoacetic acid plus 0.15 M sodium acetate at a final pH of 5.3. The solution is then heated 40 minutes at 55°C and cooled. Finally, 1 M CaCl2 is added to the solution to 5 mM. Store frozen in small aliquots (according to ref. 2 page 3.12.6 Supplement 8).