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北京西美杰科技有限公司
主营产品: 生物学检测试剂盒,化学试剂,蛋白质氧化损伤,抗原抗体 |

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A5179,0025Geno/miracle DNA Isolation Spin-Kit(DNA提取试剂盒20ug)
参考价 | 面议 |
具体成交价以合同协议为准
- 型号 A5179,0025
- 品牌
- 厂商性质 代理商
- 所在地 上海市
更新时间:2017-07-17 21:05:08浏览次数:779
联系我们时请说明是化工仪器网上看到的信息,谢谢!
usage | for 25 or 100 isolations | |||
---|---|---|---|---|
HS-No. | 38220000 | |||
Storage | RT | |||
except for Proteinase K - Solution: +4°C | ||||
• Kit for universal genomic DNA purification from difficult sources | ||||
• Applications: PCR, Southern blotting, gene library cloning | ||||
• Binding capacity per column 20 μg | ||||
• Advantages: high DNA recovery, highest purity |
This kit is based on the unique anion exchange membrane with very high affinity to DNA. Cells or tissue are Iysed with a special Lysis Solution AC1.4 and Proteinase K. In the next step the Iysate, which contains DNA, is loaded onto a spin column. Negatively charged phosphates on the backbone of DNA interact with the positive charges on the surface of the membrane. Under medium salt conditions, impurities are washed off. Then, the pure DNA is eluted under high salt conditions, desalted and concentrated by an alcohol precipitation. To make the process of precipitation easier and to avoid an accidental loss of the pellet, a blue-coloured precipitation enhancer is added.